TY  - JOUR
DA  - 2020/12/19
VL  - 8
IS  - 12
SP  - 2039
AU  - Dumortier, Corentin
AU  - Charlet, Rogatien
AU  - Bettaieb, Ali
AU  - Jawhara, Samir
TI  - H89 Treatment Reduces Intestinal Inflammation and Candida albicans Overgrowth in Mice
DO  - 10.3390/microorganisms8122039
JA  - Microorganisms
LA  - Anglais
UR  - http://hdl.handle.net/20.500.12210/40438
SN  - 2076-2607
AB  - Deregulation of the dynamic crosstalk between the gut microbiota, intestinal epithelial cells, and immune cells is critically involved in the development of inflammatory bowel disease and the overgrowth of opportunistic pathogens, including the human opportunistic fungus Candida albicans. In the present study, we assessed the effect of N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H89), a protein kinase A inhibitor, on the migration of macrophages to C. albicans through dextran sulphate sodium (DSS)-challenged Caco-2 cells. We also investigated the impact of H89 on intestinal inflammation and C. albicans clearance from the gut, and determined the diversity of the gut microbiota in a murine model of DSS-induced colitis. H89 reduced the migration of macrophages to C. albicans through DSS-challenged Caco-2 cells. In addition, H89 decreased C. albicans viability and diminished the expression of pro-inflammatory cytokines and innate immune receptors in macrophages and colonic epithelial Caco-2 cells. In mice with DSS-induced colitis, H89 attenuated the clinical and histological scores of inflammation and promoted the elimination of C. albicans from the gut. H89 administration to mice decreased the overgrowth of Escherichia coli and Enterococcus faecalis populations while Lactobacillus johnsonii populations increased significantly. Overall, H89 reduced intestinal inflammation and promoted the elimination of C. albicans from the gut.
KW  - H89
KW  - Candida albicans
KW  - Escherichia coli
KW  - Enterococcus faecalis
KW  - Lactobacillus johnsonii
KW  - microbiota
KW  - DSS
KW  - colitis
KW  - protein kinase A
PB  - MDPI AG
JO  - Microorganisms
ER  - 
TY  - JOUR
DA  - 2022/09/08
VL  - 10
IS  - 9
SP  - 1803
AU  - Charlet, Rogatien
AU  - Le Danvic, Chrystelle
AU  - Sendid, Boualem
AU  - Nagnan-Le Meillour, Patricia
AU  - Jawhara, Samir
TI  - Oleic Acid and Palmitic Acid from Bacteroides thetaiotaomicron and Lactobacillus johnsonii Exhibit Anti-Inflammatory and Antifungal Properties
DO  - 10.3390/microorganisms10091803
JA  - Microorganisms
LA  - Anglais
UR  - http://hdl.handle.net/20.500.12210/79040
SN  - 2076-2607
AB  - A decrease in populations of Bacteroides thetaiotaomicron and Lactobacillus johnsonii is observed during the development of colitis and fungal overgrowth, while restoration of these populations reduces inflammatory parameters and fungal overgrowth in mice. This study investigated the effect of two fatty acids from B. thetaiotaomicron and L. johnsonii on macrophages and Caco-2 cells, as well as their impact on the inflammatory immune response and on Candida glabrata overgrowth in a murine model of dextran sulfate sodium (DSS)-induced colitis. Oleic acid (OA) and palmitic acid (PA) from L. johnsonii and B. thetaiotaomicron were detected during their interaction with epithelial cells from colon samples. OA alone or OA combined with PA (FAs) reduced the expression of proinflammatory mediators in intestinal epithelial Caco-2 cells challenged with DSS. OA alone or FAs increased FFAR1, FFAR2, AMPK, and IL-10 expression in macrophages. Additionally, OA alone or FAs decreased COX-2, TNFα, IL-6, and IL-12 expression in LPS-stimulated macrophages. In the DSS murine model, oral administration of FAs reduced inflammatory parameters, decreased Escherichia coli and Enterococcus faecalis populations, and eliminated C. glabrata from the gut. Overall, these findings provide evidence that OA combined with PA exhibits anti-inflammatory and antifungal properties.
KW  - colitis
KW  - fatty acids
KW  - oleic acid
KW  - palmitic acid
KW  - inflammatory response
KW  - dextran sulfate sodium
KW  - Candida glabrata
KW  - Bacteroides thetaiotaomicron
KW  - Lactobacillus johnsonii
KW  - fungal overgrowth
PB  - MDPI AG
JO  - Microorganisms
ER  - 
