id,collection,Type de document,DOI,URL,Titre,Auteur(s),Titre de la revue,Numéro,Pagination,Date de publication,ISSN,Mot(s)-clé(s) en anglais,Discipline(s) HAL,Résumé en anglais,Langue,Audience,Équipe(s) de recherche,Collection
"00f0473c-5aa0-44db-b26a-a3799bef6e58","20.500.12210/33||20.500.12210/45","Article dans une revue scientifique","10.1016/j.aquatox.2017.10.009","http://hdl.handle.net/20.500.12210/18482","Maturation of Xenopus laevis oocytes under cadmium and lead exposures: Cell biology investigations","Slaby, Sylvain||Hanotel, Julie||Marchand, Guillaume||Lescuyer, Arlette||Bodart, Jean-Francois||Lepretre, Alain||Lemiere, Sebastien||Marin, Matthieu","Aquatic Toxicology","193;","105-110","2017-12","0166445X","Xenopus laevis||Oocyte||Maturation||Metal ions||Amphibians","Chimie/Chimie théorique et/ou physique||Sciences du Vivant [q-bio]/Biologie animale","Since amphibians are recognised as good models to assess the quality of environments, only few studies have dealt with the impacts of chemical contaminants on their gametes, while toxic effects at this stage will alter all the next steps of their life cycle. Therefore, we propose to investigate the oocyte maturation of Xenopus laevis in cadmium- and lead-contaminated conditions.  The impacts of cadmium and lead ions were explored on events involved in the hormone‐dependent process of maturation. In time-course experiments, cadmium, at the highest concentration, delayed and prevented the germinal vesicle breakdown. Even in the absence of progesterone this ion could also induce it. No such spontaneous maturation was observed after lead exposures. An acceleration of the process at the highest tested concentration of lead (90 μM), in presence of progesterone, was recorded. Cytological observations highlighted that cadmium exposures drove severe disturbances of meiotic spindle morphogenesis. At last, cadmium exposures altered the MAPK pathway, regarding the activation of ERK2 and RSK, but also the activation and the activity of the MPF, by disturbing the state of phosphorylation of Cdc2 and histone H3.  Xenopus laevis oocytes were affected by these metal ion exposures, notably by Cd2+. Signatures of these metal exposures on the oocyte maturation were detected. This germ cell appeared to be a relevant model to assess the effects of environmental contaminants such as metals.","Anglais","Non spécifiée","Régulation des signaux de division","Laboratoire Génie Civil et géo-Environnement (LGCgE) - ULR 4515||Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576"
