id,collection,Type de document,DOI,PMID,URL,Titre,Auteur(s),Titre de la revue,Numéro,Pagination,Éditeur,Date de publication,ISSN,Mot(s)-clé(s) en anglais,Discipline(s) HAL,Résumé en anglais,Langue,Article revu par les pairs,Audience,Vulgarisation,Autre(s) projet(s) ou source(s) de financement,Équipe(s) de recherche,Plateforme(s) de recherche,Collection
"47f4b428-3d67-40a3-b295-52fb25799200","20.500.12210/33||20.500.12210/91969","Article dans une revue scientifique","10.3390/molecules25194501","33019562","http://hdl.handle.net/20.500.12210/33804","Exploring the Potential of β-Catenin O-GlcNAcylation by Using Fluorescence-Based Engineering and Imaging","Kasprowicz, Angelina||Spriet, Corentin||Terryn, Christine||Rigolot, Vincent||Hardivillé, Stéphan||Alteen, Matthew G.||Lefebvre, Tony||Biot, Christophe","Molecules","25;19","4501","MDPI AG","2020-10-01","1420-3049","bioorthogonal chemistry||fluorescence||glycosylation||metabolic incorporation||GFP||β-catenin","Sciences du Vivant [q-bio]||Chimie/Chimie théorique et/ou physique","Monitoring glycosylation changes within cells upon response to stimuli remains challenging because of the complexity of this large family of post-translational modifications (PTMs). We developed an original tool, enabling labeling and visualization of the cell cycle key-regulator β-catenin in its O-GlcNAcylated form, based on intramolecular Förster resonance energy transfer (FRET) technology in cells. We opted for a bioorthogonal chemical reporter strategy based on the dual-labeling of β-catenin with a green fluorescent protein (GFP) for protein sequence combined with a chemically-clicked imaging probe for PTM, resulting in a fast and easy to monitor qualitative FRET assay. We validated this technology by imaging the O-GlcNAcylation status of β-catenin in HeLa cells. The changes in O-GlcNAcylation of β-catenin were varied by perturbing global cellular O-GlcNAc levels with the inhibitors of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). Finally, we provided a flowchart demonstrating how this technology is transposable to any kind of glycosylation.","Anglais","Oui","Internationale","Non","I-SITE CLICKNFRET","Chemical Glycobiology||O-GlcNAcylation, signalisation cellulaire et cycle cellulaire","Traitement de l'image et du signal pour la biologie (TISBio)","Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41||Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576"
