id,collection,Type de document,DOI,PMID,URL,Titre,Auteur(s),Titre de la revue,Nom court de la revue,Numéro,Pagination,Date de publication,ISSN,Mot(s)-clé(s) en anglais,Discipline(s) HAL,Résumé en anglais,Langue,Audience,Projet ANR,Équipe(s) de recherche,Collection
"66bb8eec-8fdf-48b1-81b6-b23326c56bf9","20.500.12210/33||20.500.12210/34","Article dans une revue scientifique","10.3390/bios5020276","26018780","http://hdl.handle.net/20.500.12210/18272","Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion","Zagorodko, Oleksandr||Bouckaert, Julie||Dumych, Tetiana||Bilyy, Rostyslav||Larroulet, Iban||Yanguas Serrano, Aritz||Alvarez Dorta, Dimitri||Gouin, Sébastien G.||Dima, Stefan-Ovidiu||Oancea, Florin||Boukherroub, Rabah||Szunerits, Sabine","Biosensors","Biosensors (Basel)","5;2","276-287","2015-05-26","2079-6374","Bacterial Adhesion||carbohydrates||shear force enhancement||Surface Plasmon Resonance||Gold||Biomechanical Phenomena||Escherichia coli||Escherichia coli (E. coli)||Biosensing Techniques||Mannose||surface plasmon resonance (SPR)||flow rate","Chimie/Chimie théorique et/ou physique","The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.","Anglais","Non spécifiée","Effets thérapeutiques de polymères mannosylés synthétiques capables de contrôler les infections chroniques ntestinales à Escherichia coli en interagissant avec la dynamique de glycosylation des cellules hôtes","Computational Molecular Systems Biology","Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520||Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576"
"35245f93-5325-4031-932d-b64d69cc34dc","20.500.12210/33","Article dans une revue scientifique","10.1128/mBio.01298-15","26578673","http://hdl.handle.net/20.500.12210/18269","Development of Heptylmannoside-Based Glycoconjugate Antiadhesive Compounds against Adherent-Invasive Escherichia coli Bacteria Associated with Crohn's Disease","Sivignon, Adeline||Yan, Xibo||Alvarez Dorta, Dimitri||Bonnet, Richard||Bouckaert, Julie||Fleury, Etienne||Bernard, Julien||Gouin, Sébastien G.||Darfeuille-Michaud, Arlette||Barnich, Nicolas","mBio","MBio","6;6","e01298-01215","2015-11-17","2150-7511","Intestinal Mucosa||Fimbriae Proteins||Fimbriae, Bacterial||Mannosides||Intestines||Escherichia coli Infection||Glycoconjugates||Adhesins, Escherichia coli||Administration, Oral||Cell Adhesion Molecules||Escherichia coli||GPI-Linked Proteins||Crohn Disease||Epithelial Cells||Bacterial Load||Drug Discovery||Bacterial Adhesion||Animals||beta-Cyclodextrins||Mice||Feces||Antigens, CD","Chimie/Chimie théorique et/ou physique","The ileal lesions of Crohn's disease (CD) patients are colonized by adherent-invasive Escherichia coli (AIEC) bacteria. These bacteria adhere to mannose residues expressed by CEACAM6 on host cells in a type 1 pilus-dependent manner. In this study, we investigated different antagonists of FimH, the adhesin of type 1 pili, for their ability to block AIEC adhesion to intestinal epithelial cells (IEC). Monovalent and multivalent derivatives of n-heptyl α-d-mannoside (HM), a nanomolar antagonist of FimH, were tested in vitro in IEC infected with the AIEC LF82 strain and in vivo by oral administration to CEACAM6-expressing mice infected with LF82 bacteria. In vitro, multivalent derivatives were more potent than the monovalent derivatives, with a gain of efficacy superior to their valencies, probably owing to their ability to form bacterial aggregates. Of note, HM and the multi-HM glycoconjugates exhibited lower efficacy in vivo in decreasing LF82 gut colonization. Interestingly, HM analogues functionalized with an isopropylamide (1A-HM) or β-cyclodextrin pharmacophore at the end of the heptyl tail (1CD-HM) exerted beneficial effects in vivo. These two compounds strongly decreased the amount of LF82 bacteria in the feces of mice and that of bacteria associated with the gut mucosa when administered orally at a dose of 10 mg/kg of body weight after infection. Importantly, signs of colitis and intestinal inflammation induced by LF82 infection were also prevented. These results highlight the potential of the antiadhesive compounds to treat CD patients abnormally colonized by AIEC bacteria and point to an alternative to the current approach focusing on blocking proinflammatory mediators. IMPORTANCE: Current treatments for Crohn's disease (CD), including immunosuppressive agents, anti-tumor necrosis factor alpha (anti-TNF-α) and anti-integrin antibodies, focus on the symptoms but not on the cause of the disease. Adherent-invasive Escherichia coli (AIEC) bacteria abnormally colonize the ileal mucosa of CD patients via the interaction of the mannose-specific adhesin FimH of type 1 pili with CEACAM6 mannosylated proteins expressed on the epithelial cell surface. Thus, we decided to develop an antiadhesive strategy based on synthetic FimH antagonists specifically targeting AIEC bacteria that would decrease intestinal inflammation. Heptylmannoside (HM)-based glycocompounds strongly inhibit AIEC adhesion to intestinal epithelial cells in vitro. The antiadhesive effect of two of these compounds of relatively simple chemical structure was also observed in vivo in AIEC-infected CEACAM6-expressing mice and was associated with a reduction in the signs of colitis. These results suggest a new therapeutic approach for CD patients colonized by AIEC bacteria, based on the development of synthetic FimH antagonists.","Anglais","Non spécifiée","Effets thérapeutiques de polymères mannosylés synthétiques capables de contrôler les infections chroniques ntestinales à Escherichia coli en interagissant avec la dynamique de glycosylation des cellules hôtes","Computational Molecular Systems Biology","Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576"
"46473b01-bca9-477e-a4b5-33c5e41d142b","20.500.12210/33||20.500.12210/34","Article dans une revue scientifique","10.1016/j.snb.2017.12.169",,"http://hdl.handle.net/20.500.12210/18577","Reduced graphene oxide/polyethylenimine based immunosensor for the selective and sensitive electrochemical detection of uropathogenic Escherichia coli","Jijie, Roxana||Kahlouche, Karima||Barras, Alexandre||Yamakawa, Nao||Bouckaert, Julie||Gharbi, Tijani||Szunerits, Sabine||Boukherroub, Rabah","Sensors and Actuators B. Chemical",,"260;","255-263","2018-05","09254005","Electrophoretic deposition||Reduced graphene oxide||Polyethyleneimine||Anti- fimbrial E. coli antibodies||E. coli UTI89 bacteria||Electrochemical sensing","Chimie/Chimie théorique et/ou physique","Fast, reliable and selective detection of microorganisms is of uttermost importance in clinical analysis, but also in food and water quality monitoring. In this study, we report on the construction of an immunosensor for sensitive and selective electrochemical detection of uropathogenic Escherichia coli (E. coli) UTI89 bacteria in aqueous and serum samples. We took benefit of electrophoretic deposition (EPD) to prepare, in a simple, controllable and cost effective way, gold electrodes modified with thin active layers of reduced graphene oxide/polyethylenimine (rGO/PEI). While rGO exhibits high surface area and favourable electrochemical properties, the presence of abundant single bondNH2 groups on PEI offers a plethora of opportunities for the sensor’s surface functionalization. To achieve selectivity of detection, the electrode surface was covalently modified with anti-fimbrial E. coli antibodies via amide bond formation. To minimize non-specific adsorption, the immunosensor was additionally modified with pyrene-polyethyleneglycol (pyrene-PEG) moieties prior to antibody immobilization. The detection of E. coli was based on the restriction of electron transfer of a redox mediator, in our case potassium ferrocyanide, to the rGO/PEI modified electrical transducer due to the formation of an immune complex. The developed immunosensor displayed a sigmoidal shape with a linear range of 1 × 101–1 × 104 cfu mL−1 (R2 = 0.995) according to i(μA) = −16.66–20.5 × log[E. coli] (cfu mL−1) and a detection limit of 10 cfu mL−1. Additionally, the sensor performed well both in aqueous, serum and urine media, which is essential for its potential use for clinical diagnosis of pathogenic diseases. Selectivity studies showed that the immunosensor was able to discriminate between E. coli UTI89 wild-type strain and UTI89 Δfim, without fim operon.","Anglais","Non spécifiée","Effets thérapeutiques de polymères mannosylés synthétiques capables de contrôler les infections chroniques ntestinales à Escherichia coli en interagissant avec la dynamique de glycosylation des cellules hôtes","Computational Molecular Systems Biology","Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520||Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576"
"14e2f31c-d4ae-46dc-8474-f4555be1de64","20.500.12210/33","Article dans une revue scientifique","10.1007/978-1-4939-1292-6_42","25117261","http://hdl.handle.net/20.500.12210/18296","Small-angle X-ray scattering to obtain models of multivalent lectin-glycan complexes","Weeks, Stephen D.||Bouckaert, Julie","Methods in molecular biology (Clifton, N.J.)","Methods Mol. Biol.","1200;","511-526","2014-07-22","1940-6029","Protein Structure, Tertiary||Animals||Protein Structure, Secondary||Cattle||X-Ray Diffraction||Models, Molecular||Scattering, Small Angle||Lectins||Polysaccharide||Bacterial Proteins","Chimie/Chimie théorique et/ou physique","Recent advances in small-angle X-ray scattering (SAXS) have led to the ability to model the glycans on glycoproteins and to obtain the low-resolution solution structures of complexes of lectins bound to multivalent glycan-presenting scaffolds. This progress in SAXS can respond to the increasing interest in the biological action of glycoproteins and lectins and in the design of multivalent glycan-based antagonists. Carbohydrates make up a significant part of the X-ray scattering content in SAXS and should be included in the model together with the protein, whose structure is most often based on a crystal structure or NMR ensemble, to give a far-improved fit with the experimental data. The modeling of the spatial positioning of glycans on proteins or in the architecture of lectin-glycan complexes delivers low-resolution structural information hitherto unmatched by any other method. SAXS data on the bacterial lectin FimH, strongly bound to heptyl α-D-mannose on a sevenfold derivatized β-cyclodextrin, permitted determination of the stoichiometry of the complex and the geometry of the lectin deposition on the multivalent β-cyclodextrin. The SAXS methods can be applied to larger complexes as the technique imposes no limit on the size of the macromolecular assembly in solution.","Anglais","Non spécifiée","Effets thérapeutiques de polymères mannosylés synthétiques capables de contrôler les infections chroniques ntestinales à Escherichia coli en interagissant avec la dynamique de glycosylation des cellules hôtes","Computational Molecular Systems Biology","Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576"
