id,collection,Type de document,DOI,URL,Titre,Auteur(s),Titre de la revue,Numéro,Pagination,Date de publication,ISSN,Discipline(s) HAL,Résumé en anglais,Langue,Audience,Équipe(s) de recherche,Collection
"18105201-98e3-46f0-ae00-c53ce66c385e","20.500.12210/33","Article dans une revue scientifique","10.1371/journal.pone.0136048","http://hdl.handle.net/20.500.12210/18520","Spatial Localization and Binding of the Probiotic Lactobacillus farciminis to the Rat Intestinal Mucosa: Influence of Chronic Stress","Da Silva, Stéphanie||Masselot, Catherine||Raymond, Arthur||Mercade-Loubière, Myriam||Salvador-Cartier, Christel||Ringot-Destrez, Bélinda||Leonard, Renaud||Fourquaux, Isabelle||Ait-Belgnaoui, Afifa||Loubière, Pascal||Théodorou, Vassilia||Mercier-Bonin, Muriel","PLoS One","10;9","e0136048","2015-09-14","1932-6203","Chimie/Chimie théorique et/ou physique||Sciences du Vivant [q-bio]/Ingénierie des aliments","The present study aimed at detecting the exogenously applied probiotic Lactobacillus farciminis in rats, after exposure to IBS-like chronic stress, based on 4-day Water Avoidance Stress (WAS). The presence of L. farciminis in both ileal and colonic mucosal tissues was demonstrated by FISH and qPCR, with ileum as the preferential niche, as for the SFB population. A different spatial distribution of the probiotic was observed: in the ileum, bacteria were organized in micro-colonies more or less close to the epithelium whereas, in the colon, they were mainly visualized far away from the epithelium. When rats were submitted to WAS, the L. farciminis population substantially decreased in both intestinal regions, due to a stress-induced increase in colonic motility and defecation, rather than a modification of bacterial binding to the intestinal mucin Muc2.","Anglais","Non spécifiée","Génétique des enveloppes bactériennes","Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576"
