Deciphering the transcriptional landscape ...
Document type :
Article dans une revue scientifique: Article original
DOI :
PMID :
Permalink :
Title :
Deciphering the transcriptional landscape of human pluripotent stem cell-derived GnRH neurons: the role of Wnt signaling in patterning the neural fate.
Author(s) :
Wang, Y. [Auteur]
Madhusudan, S. [Auteur]
Cotellessa, Ludovica [Auteur]
Lille Neurosciences & Cognition - U 1172 [LilNCog]
Kvist, J. [Auteur]
Eskici, N. [Auteur]
Yellapragada, V. [Auteur]
Pulli, K. [Auteur]
Lund, C. [Auteur]
Vaaralahti, K. [Auteur]
Tuuri, T. [Auteur]
Giacobini, Paolo [Auteur]
Lille Neurosciences & Cognition (LilNCog) - U 1172
Raivio, Taneli [Auteur]
Helsingin yliopisto = Helsingfors universitet = University of Helsinki
Madhusudan, S. [Auteur]
Cotellessa, Ludovica [Auteur]
Lille Neurosciences & Cognition - U 1172 [LilNCog]
Kvist, J. [Auteur]
Eskici, N. [Auteur]
Yellapragada, V. [Auteur]
Pulli, K. [Auteur]
Lund, C. [Auteur]
Vaaralahti, K. [Auteur]
Tuuri, T. [Auteur]
Giacobini, Paolo [Auteur]

Lille Neurosciences & Cognition (LilNCog) - U 1172
Raivio, Taneli [Auteur]
Helsingin yliopisto = Helsingfors universitet = University of Helsinki
Journal title :
Stem Cells
Volume number :
40
Pages :
1107–1121
Publisher :
Oxford University Press
Publication date :
2022-09-29
ISSN :
1549-4918
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Hypothalamic gonadotropin-releasing hormone (GnRH) neurons lay the foundation for human development and reproduction; however, the critical cell populations and the entangled mechanisms underlying the development of human ...
Show more >Hypothalamic gonadotropin-releasing hormone (GnRH) neurons lay the foundation for human development and reproduction; however, the critical cell populations and the entangled mechanisms underlying the development of human GnRH neurons remain poorly understood. Here, by using our established human pluripotent stem cell-derived GnRH neuron model, we decoded the cellular heterogeneity and differentiation trajectories at the single-cell level. We found that a glutamatergic neuron population, which generated together with GnRH neurons, showed similar transcriptomic properties with olfactory sensory neuron and provided the migratory path for GnRH neurons. Through trajectory analysis, we identified a specific gene module activated along the GnRH neuron differentiation lineage, and we examined one of the transcription factors, DLX5, expression in human fetal GnRH neurons. Furthermore, we found that Wnt inhibition could increase DLX5 expression and improve the GnRH neuron differentiation efficiency through promoting neurogenesis and switching the differentiation fates of neural progenitors into glutamatergic neurons/GnRH neurons. Our research comprehensively reveals the dynamic cell population transition and gene regulatory network during GnRH neuron differentiation.Show less >
Show more >Hypothalamic gonadotropin-releasing hormone (GnRH) neurons lay the foundation for human development and reproduction; however, the critical cell populations and the entangled mechanisms underlying the development of human GnRH neurons remain poorly understood. Here, by using our established human pluripotent stem cell-derived GnRH neuron model, we decoded the cellular heterogeneity and differentiation trajectories at the single-cell level. We found that a glutamatergic neuron population, which generated together with GnRH neurons, showed similar transcriptomic properties with olfactory sensory neuron and provided the migratory path for GnRH neurons. Through trajectory analysis, we identified a specific gene module activated along the GnRH neuron differentiation lineage, and we examined one of the transcription factors, DLX5, expression in human fetal GnRH neurons. Furthermore, we found that Wnt inhibition could increase DLX5 expression and improve the GnRH neuron differentiation efficiency through promoting neurogenesis and switching the differentiation fates of neural progenitors into glutamatergic neurons/GnRH neurons. Our research comprehensively reveals the dynamic cell population transition and gene regulatory network during GnRH neuron differentiation.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
Inserm
CHU Lille
Inserm
CHU Lille
Collections :
Submission date :
2024-01-16T00:39:56Z
2024-09-11T08:13:02Z
2024-09-11T08:13:02Z
Files
- sxac069.pdf
- Non spécifié
- Open access
- Access the document