In vitro differentiation modifies the neurotoxic response of SH-SY5Y cells.

De Conto, Véronique; Cheung, V.; Maubon, G.; Souguir, Z.; Maubon, N.; Vandenhaute, E.; Berezowski, Vincent

Document type :

Article dans une revue scientifique: Article original

DOI :

10.1016/j.tiv.2021.105235

PMID :

34425233

Permalink :

http://hdl.handle.net/20.500.12210/102054

Title :

In vitro differentiation modifies the neurotoxic response of SH-SY5Y cells.

Author(s) :

De Conto, Véronique [Auteur]
Lille Neurosciences & Cognition - U 1172 [LilNCog]
Cheung, V. [Auteur]
Maubon, G. [Auteur]
Souguir, Z. [Auteur]
Maubon, N. [Auteur]
Vandenhaute, E. [Auteur]
Berezowski, Vincent [Auteur]

Lille Neurosciences & Cognition (LilNCog) - U 1172

Journal title :

Toxicology in Vitro

Abbreviated title :

Toxicol In Vitro

Volume number :

Pages :

105235

Publisher :

Elsevier

Publication date :

2021-12-01

ISSN :

1879-3177

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The SH-SY5Y cell line is commonly used for the assessment of neurotoxicity in drug discovery. These neuroblastoma-derived cells can be differentiated into neurons using many methods. The present study has compared 24 of ...
Show more >The SH-SY5Y cell line is commonly used for the assessment of neurotoxicity in drug discovery. These neuroblastoma-derived cells can be differentiated into neurons using many methods. The present study has compared 24 of these differentiation methods on SH-SY5Y cells. After morphologic selection of the three most differentiating media (retinoic acid in 10% fetal bovine serum (FBS), staurosporine in 1% FBS medium, and cyclic adenosine monophosphate (cAMP) in B21-supplemented neurobasal medium), cells were analyzed for pan-neuronal and specific neuronal protein expression by fluorescent automated imaging. The response of SH-SY5Y to a set of compounds of known toxicity was examined in these culture conditions performed in 2D, and also in a 3D hyaluronic acid-based hydroscaffold™ which mimics the extracellular matrix. The extent of neuronal markers expression and the sensitivity to neurotoxic compounds varied according to the differentiation medium. The cAMP B21-supplemented neurobasal medium led to the higher neuronal differentiation, and the higher sensitivity to neurotoxic compounds. The culture in 3D modified the neurotoxic response, through a lower sensitivity of cells compared to the 2D culture. The in vitro differentiation environment influences the neurotoxic response of SH-SY5Y cells and thus should be considered carefully in research as well as in drug discovery.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
Inserm
CHU Lille

Collections :

Lille Neurosciences & Cognition (LilNCog) - U 1172

Submission date :

2024-01-16T01:51:02Z
2024-11-06T10:52:40Z

Version	Link	Modification date
2	20.500.12210/102054*	2024-11-06T10:48:20Z
1	20.500.12210/102054.1	2024-01-16T01:51:02Z

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