Title :

Ku86 is important for TrkA overexpression-induced breast cancer cell invasion

Author(s) :

Lagadec, Chann [Auteur]
Romon, Rodrigue [Auteur]
Tastet, Christophe [Auteur]
Meignan, Samuel [Auteur]
Com, Emmanuelle [Auteur]
Page, Adeline [Auteur]
Bidaux, Gabriel [Auteur]
Cardiovasculaire, métabolisme, diabétologie et nutrition [CarMeN]
Hondermarck, Hubert [Auteur]
Le Bourhis, Xuefen [Auteur]

Journal title :

PROTEOMICS - Clinical Applications

Pages :

580-590

Publisher :

Wiley-VCH Verlag

Publication date :

2010-07

ISSN :

1862-8346

English keyword(s) :

RPLP0
human acidic ribosomal phosphoprotein P0
siRNA
smallinterfering RNA
TRAIL
TNF-related apoptosis inducing ligand breast cancer / Ku proteins / cell invasion / TrkA tyrosine kinase receptor

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Purpose: We have recently shown that breast tumors express high levels of TrkA compared with normal breast tissues, with TrkA overexpression enhancing breast cancer cell invasion in vitro and metastasis in animal models. ...
Show more >Purpose: We have recently shown that breast tumors express high levels of TrkA compared with normal breast tissues, with TrkA overexpression enhancing breast cancer cell invasion in vitro and metastasis in animal models. In this study, we tried to identify molecules involved in TrkA overexpression-mediated biological effects in breast cancer cellsExperimental design: We used a proteomic-based approach to identify proteins involved in TrkA overexpression-stimulated invasion of MDA-MB-231 breast cancer cells. Proteins from control and TrkA overexpressing cells were separated using a cup-loading two-dimensional electrophoresis system before MALDI and LC-MS/MS mass spectrometry analysisResults Among several putative regulated proteins, Ku86 was found increased in TrkA overexpressing cells. Moreover, Ku86 was co-immunoprecipitated with TrkA, suggesting the interaction of these two proteins in TrkA overexpressing cells. Interestingly, inhibition with small-interfering RNA and neutralizing antibodies showed that Ku86 was required for TrkA-stimulated cell invasionConclusions and clinical relevance. These data allowed the identification of Ku86 as a new player involved in metastasis in breast cancer cells. Our findings suggest that TrkA and its down stream signaling pathways should be regarded as potential new targets for the development of future breast cancer therapyShow less >

Language :

Anglais

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL

Submission date :

2024-02-17T04:27:28Z