Proteogenomic Insights into the Intestinal Parasite Blastocystis sp. Subtype 4 Isolate WR1

Armengaud, Jean; Pible, Olivier; Gaillard, Jean‐charles; Cian, Amandine; Gantois, Nausicaa; Tan, Kevin; Chabé, Magali; Viscogliosi, Eric

Document type :

Autre communication scientifique (congrès sans actes - poster - séminaire...)

DOI :

10.1002/pmic.201700211

Title :

Proteogenomic Insights into the Intestinal Parasite Blastocystis sp. Subtype 4 Isolate WR1

Author(s) :

Armengaud, Jean [Auteur]
Pible, Olivier [Auteur]
Gaillard, Jean‐charles [Auteur]
Cian, Amandine [Auteur]
Gantois, Nausicaa [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Tan, Kevin [Auteur]
Chabé, Magali [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Viscogliosi, Eric [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]

Publisher :

Wiley-VCH Verlag

Publication date :

2017-10-16

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Abstract Blastocystis sp. is known for years as a highly prevalent anaerobic eukaryotic parasite of humans and animals. Several monophyletic clades have been delineated based on molecular data, and the occurrence of each ...
Show more >Abstract Blastocystis sp. is known for years as a highly prevalent anaerobic eukaryotic parasite of humans and animals. Several monophyletic clades have been delineated based on molecular data, and the occurrence of each subtype in humans and/or animal hosts has been documented. The genome of several representatives has been sequenced revealing specific traits such as an intriguing 3'‐end processing of primary transcripts. Here, a first high‐throughput proteomics dataset acquired on this difficult‐to‐cultivate parasite is presented for the zoonotic subtype T4 isolate WR1. Amongst the 2766 detected proteins, we highlighted the role of a small ADP ribosylation factor GTP‐binding protein involved in intracellular traffic as major regulator of vesicle biogenesis and a voltage‐dependent anion‐selective channel protein because both were unexpectedly highly abundant. We show how these data may be used for gaining proteogenomics insights into Blastocystis sp. specific molecular mechanisms. We evidenced for the first time by proteogenomics a functional termination codon derived from transcript polyadenylation for seven different key cellular components.Show less >

Language :

Anglais

Popular science :

Non

Collections :

Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL

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