Title :

The phosphatidylserine receptor TIM1 promotes infection of enveloped hepatitis E virus

Author(s) :

Corneillie, Laura [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Lemmens, Irma [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Montpellier, Claire [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Ferrié, Martin [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Weening, Karin [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
van Houtte, Freya [Auteur]
Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 [RID-AGE]
Hanoulle, Xavier [Auteur]
Cocquerel, Laurence [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Amara, Ali [Auteur]
Génomes, biologie cellulaire et thérapeutiques [GenCellDis (U944 / UMR7212)]
Tavernier, Jan [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Meuleman, Philip [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]

Journal title :

Cellular and Molecular Life Sciences

Pages :

326

Publisher :

Springer Verlag

Publication date :

2023

ISSN :

1420-682X

English keyword(s) :

Hepatitis E virus HEV
TIM1
Virus-host cell interaction
Viral entry
Cell surface receptor
Apoptotic mimicry

HAL domain(s) :

Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Maladies infectieuses
Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Hépatologie et Gastroentérologie
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie

English abstract : [en]

The hepatitis E virus (HEV) is an underestimated RNA virus of which the viral life cycle and pathogenicity remain partially understood and for which specific antivirals are lacking. The virus exists in two forms: nonenveloped ...
Show more >The hepatitis E virus (HEV) is an underestimated RNA virus of which the viral life cycle and pathogenicity remain partially understood and for which specific antivirals are lacking. The virus exists in two forms: nonenveloped HEV that is shed in feces and transmits between hosts; and membrane-associated, quasi-enveloped HEV that circulates in the blood. It is suggested that both forms employ different mechanisms for cellular entry and internalization but little is known about the exact mechanisms. Interestingly, the membrane of enveloped HEV is enriched with phosphatidylserine, a natural ligand for the T-cell immunoglobulin and mucin domain-containing protein 1 (TIM1) during apoptosis and involved in ‘apoptotic mimicry’, a process by which viruses hijack the apoptosis pathway to promote infection. We here investigated the role of TIM1 in the entry process of HEV. We determined that HEV infection with particles derived from culture supernatant, which are cloaked by host-derived membranes (eHEV), was significantly impaired after knockout of TIM1, whereas infection with intracellular HEV particles (iHEV) was unaffected. eHEV infection was restored upon TIM1 expression; and enhanced after ectopic TIM1 expression. The significance of TIM1 during entry was further confirmed by viral binding assay, and point mutations of the PS-binding pocket diminished eHEV infection. In addition, Annexin V, a PS-binding molecule also significantly reduced infection. Taken together, our findings support a role for TIM1 in eHEV-mediated cell entry, facilitated by the PS present on the viral membrane, a strategy HEV may use to promote viral spread throughout the infected body.Show less >

Language :

Anglais

Popular science :

Non

Collections :

• Facteurs de risque et déterminants moléculaires des maladies liées au vieillissement (RID-AGE) - U1167

Source :

Harvested from HAL