Title :

In vitro studies of human and rat osteoclast activity on hydroxyapatite, b-tricalcium phosphate, calcium carbonate

Author(s) :

Monchau, Francine [Auteur]
Lefèvre, A [Auteur]
Descamps, Michel [Auteur]
Laboratoire des Matériaux Céramiques et Procédés Associés - EA 2443 [LMCPA]
Belquin-Myrdycz C, A [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Laffargue, P [Auteur]
Hildebrand, H [Auteur]

Journal title :

Biomolecular Engineering

Pages :

143-152

Publisher :

Elsevier

Publication date :

2002

ISSN :

1389-0344

English keyword(s) :

Human osteoclasts
Rat osteoclasts
Resorption
Bioceramics
Hydroxyapatite
b-tricalcium phosphate
Calcium carbonate

HAL domain(s) :

Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Ingénierie biomédicale/Biomatériaux

English abstract : [en]

Investigations on the ceramic degradation caused by osteoclasts are designed to assess osteoclast Á/ceramic interactions and to determine which ceramics are more suitable for use as bone substitute. This study investigated ...
Show more >Investigations on the ceramic degradation caused by osteoclasts are designed to assess osteoclast Á/ceramic interactions and to determine which ceramics are more suitable for use as bone substitute. This study investigated the resorptive activity of osteoclasts on ceramics presenting different solubility rates. Osteoclasts isolated from newborn rat and from human giant cell tumour were cultured on different bioceramics: hydroxyapatite (HA), b-tricalcium phosphate (TCP) and calcium carbonate (calcite). Cytoskeletal was revealed by actin labelling and ceramic surfaces were observed by scanning electron microscopy (SEM). On all materials, the distribution of actin in typical ring was revealed. SEM examinations showed a clear difference in the shape and the depth of resorption lacunae on different ceramics. On pure HA, a superficial attack, clearly visible but very little extended. Numerous resorption lacunae, deep and well-delimited were observed on pure b-TCP, but attacks less punctually were detected too. On pure calcite, an attack with form of spikes, very widespread but superficial was revealed. Degradation measurements revealed a significant increase of P release from the phosphocalcic ceramics and of Ca from all ceramics in the presence of osteoclasts. The both cell models found these characteristics, the rat osteoclasts were also an excellent model to study the ceramic resorption.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520

Source :

Harvested from HAL