Title :

Large dynamics of a phase separating arginine-glycine-rich domain revealed via nuclear and electron spins.

Author(s) :

Sicoli, Giuseppe [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516
Sieme, Daniel [Auteur]
Max Planck Institute for Multidisciplinary Sciences / Max-Planck-Institut für Multidisziplinäre Naturwissenschaften [Göttingen, Germany]
Overkamp, Kerstin [Auteur]
Max Planck Institute for Multidisciplinary Sciences / Max-Planck-Institut für Multidisziplinäre Naturwissenschaften [Göttingen, Germany]
Khalil, Mahdi [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516
Backer, Robin [Auteur]
Faculty of Mathematics and Natural Sciences
Griesinger, Christian [Auteur]
Max Planck Institute for Biophysical Chemistry [MPI-BPC]
Willbold, Dieter [Auteur]
Faculty of Mathematics and Natural Sciences
Rezaei-Ghaleh, Nasrollah [Auteur]
Faculty of Mathematics and Natural Sciences

Journal title :

Nat Commun

Abbreviated title :

Nat Commun

Volume number :

15

Pages :

1610

Publication date :

2024-02-23

ISSN :

2041-1723

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Liquid-liquid phase separation is the key process underlying formation of membrane-less compartments in cells. A highly dynamic cellular body with rapid component exchange is Cajal body (CB), which supports the extensive ...
Show more >Liquid-liquid phase separation is the key process underlying formation of membrane-less compartments in cells. A highly dynamic cellular body with rapid component exchange is Cajal body (CB), which supports the extensive compositional dynamics of the RNA splicing machinery, spliceosome. Here, we select an arginine-glycine (RG)-rich segment of coilin, the major component of CB, establish its RNA-induced phase separation, and through combined use of nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) probes, interrogate its dynamics within the crowded interior of formed droplets. Taking advantage of glycine-based singlet-states, we show that glycines retain a large level of sub-nanoseconds dynamics inside the coilin droplets. Furthermore, the continuous-wave (CW) and electron-electron dipolar (PELDOR) and electron-nucleus hyperfine coupling EPR data (HYSCORE) support the RNA-induced formation of dynamic coilin droplets with high coilin peptide concentrations. The combined NMR and EPR data reveal the high dynamics of the RG-rich coilin within droplets and suggest its potential role in the large dynamics of CBs.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516

Research team(s) :

Propriétés magnéto structurales des matériaux (PMSM)

Submission date :

2024-02-28T22:03:00Z
2024-03-13T12:23:36Z