Title :

Per-pixel unmixing of spectrally overlapping fluorophores using intra-exposure excitation modulation.

Author(s) :

Valenta, H. [Auteur]
Bierbuesse, F. [Auteur]
Vitale, Raffaele [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516
Ruckebusch, Cyril [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516
Vandenberg, W. [Auteur]
Dedecker, P. [Auteur]

Journal title :

Talanta

Abbreviated title :

Talanta

Volume number :

269

Pages :

125397

Publication date :

2023-12-05

ISSN :

1873-3573

English keyword(s) :

Multiplexed imaging
Per-pixel unmixing
Reversibly photoswitchable fluorescent
proteins

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Multilabel fluorescence imaging is essential for the visualization of complex systems, though a major challenge is the limited width of the useable spectral window. Here, we present a new method, exNEEMO, that enables ...
Show more >Multilabel fluorescence imaging is essential for the visualization of complex systems, though a major challenge is the limited width of the useable spectral window. Here, we present a new method, exNEEMO, that enables per-pixel quantification of spectrally-overlapping fluorophores based on their light-induced dynamics, in a way that is compatible with a very broad range of timescales over which these dynamics may occur. Our approach makes use of intra-exposure modulation of the excitation light to distinguish the different emitters given their reference responses to this modulation. We use the approach to simultaneously image four green photochromic fluorescent proteins at the full spatial resolution of the imaging.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516

Submission date :

2024-02-28T22:05:04Z
2024-03-11T15:50:38Z