Genetic Engineering of Zebrafish in Cancer Research

Raby, Ludivine; Völkel, Pamela; Le Bourhis, Xuefen; Angrand, Pierre-Olivier

Type de document :

Article dans une revue scientifique: Article original

DOI :

10.3390/cancers12082168

Titre :

Genetic Engineering of Zebrafish in Cancer Research

Auteur(s) :

Raby, Ludivine [Auteur]
Völkel, Pamela [Auteur]
Le Bourhis, Xuefen [Auteur]

Angrand, Pierre-Olivier [Auteur correspondant]

Université de Lille
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]

Titre de la revue :

Cancers

Pagination :

2168

Éditeur :

MDPI

Date de publication :

2020-08-04

ISSN :

2072-6694

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Zebrafish (Danio rerio) is an excellent model to study a wide diversity of human cancers. In this review, we provide an overview of the genetic and reverse genetic toolbox allowing the generation of zebrafish lines that ...
Lire la suite >Zebrafish (Danio rerio) is an excellent model to study a wide diversity of human cancers. In this review, we provide an overview of the genetic and reverse genetic toolbox allowing the generation of zebrafish lines that develop tumors. The large spectrum of genetic tools enables the engineering of zebrafish lines harboring precise genetic alterations found in human patients, the generation of zebrafish carrying somatic or germline inheritable mutations or zebrafish showing conditional expression of the oncogenic mutations. Comparative transcriptomics demonstrate that many of the zebrafish tumors share molecular signatures similar to those found in human cancers. Thus, zebrafish cancer models provide a unique in vivo platform to investigate cancer initiation and progression at the molecular and cellular levels, to identify novel genes involved in tumorigenesis as well as to contemplate new therapeutic strategies.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :