Title :

Genetic architecture of a plant adaptive trait: QTL mapping of intraspecific variation for tolerance to metal pollution in Arabidopsis halleri

Author(s) :

Karam, Marie-Joe [Auteur]
Université de Lille
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Souleman, Dima [Auteur]
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Université de Lille
Schvartzman, M. Sol [Auteur]
Integrative Biological Sciences [InBioS]
Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich [ULiège]
Gallina, Sophie [Auteur]
Université de Lille
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Spielmann, Julien [Auteur]
Integrative Biological Sciences [InBioS]
Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich [ULiège]
Poncet, Charles [Auteur]
Génétique Diversité et Ecophysiologie des Céréales [GDEC]
Bouchez, Olivier [Auteur]
Génome et Transcriptome - Plateforme Génomique [GeT-PlaGe]
Plateforme Bio-Informatique - Génotoul
Pauwels, Maxime [Auteur]
Université de Lille
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Hanikenne, Marc [Auteur]
Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich [ULiège]
Integrative Biological Sciences [InBioS]
Frérot, Hélène [Auteur]
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Université de Lille

Journal title :

Heredity

Pages :

877-892

Publisher :

Nature Publishing Group

Publication date :

2019-01-22

ISSN :

0018-067X

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Anthropogenic activities are among the main drivers of global change and result in drastic habitat modifications, which represent strong evolutionary challenges for biological species that can either migrate, adapt, or ...
Show more >Anthropogenic activities are among the main drivers of global change and result in drastic habitat modifications, which represent strong evolutionary challenges for biological species that can either migrate, adapt, or disappear. In this context, understanding the genetics of adaptive traits is a prerequisite to enable long-term maintenance of populations under strong environmental constraints. To examine these processes, a QTL approach was developed here using the pseudometallophyte Arabidopsis halleri, which displays among-population adaptive divergence for tolerance to metallic pollution in soils. An F2 progeny was obtained by crossing individuals from metallicolous and non-metallicolous populations from Italian Alps, where intense metallurgic activities have created strong landscape heterogeneity. Then, we combined genome de novo assembly and genome resequencing of parental genotypes to obtain single-nucleotide polymorphism markers and achieve high-throughput genotyping of the progeny. QTL analysis was performed using growth parameters and photosynthetic yield to assess zinc tolerance levels. One major QTL was identified for photosynthetic yield. It explained about 27% of the phenotypic variance. Functional annotation of the QTL and gene expression analyses highlighted putative candidate genes. Our study represents a successful approach combining evolutionary genetics and advanced molecular tools, helping to better understand how a species can face new selective pressures of anthropogenic origin.Show less >

Language :

Anglais

Popular science :

Non

ANR Project :

Evolution de l'adaptation locale en environnement anthropisé

Collections :

• Évolution, Écologie et Paléontologie (Evo Eco Paleo) - UMR 8198

Source :

Harvested from HAL