Divergent Binding and Transactivation by ...
Document type :
Article dans une revue scientifique
DOI :
PMID :
Permalink :
Title :
Divergent Binding and Transactivation by Two Related Steroid Receptors at the Same Response Element
Author(s) :
Tesikova, Martina [Auteur]
Dezitter, Xavier [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Nenseth, Hatice Z. [Auteur]
Klokk, Tove I. [Auteur]
Mueller, Florian [Auteur]
Hager, Gordon L. [Auteur]
Saatcioglu, Fahri [Auteur]
Dezitter, Xavier [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Nenseth, Hatice Z. [Auteur]
Klokk, Tove I. [Auteur]
Mueller, Florian [Auteur]
Hager, Gordon L. [Auteur]
Saatcioglu, Fahri [Auteur]
Journal title :
The Journal of biological chemistry
Abbreviated title :
J. Biol. Chem.
Volume number :
291
Pages :
11899-11910
Publication date :
2016-05-27
ISSN :
0021-9258
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Transcription factor (TF) recruitment to chromatin is central to activation of transcription. TF-chromatin interactions are highly dynamic, which are evaluated by recovery half time (t1/2) in seconds, determined by ...
Show more >Transcription factor (TF) recruitment to chromatin is central to activation of transcription. TF-chromatin interactions are highly dynamic, which are evaluated by recovery half time (t1/2) in seconds, determined by fluorescence recovery experiments in living cells, and chromatin immunoprecipitation (ChIP) analysis, measured in minutes. These two states are related: the larger the t1/2, the longer the ChIP occupancy resulting in increased transcription. Here we present data showing that this relationship does not always hold. We found that histone deacetylase inhibitors (HDACis) significantly increased t1/2 of green fluorescent protein (GFP) fused androgen receptor (AR) on a tandem array of positive hormone response elements (HREs) in chromatin. This resulted in increased ChIP signal of GFP-AR. Unexpectedly, however, transcription was inhibited. In contrast, the GFP-fused glucocorticoid receptor (GR), acting through the same HREs, displayed a profile consistent with current models. We provide evidence that these differences are mediated, at least in part, by HDACs. Our results provide insight into TF action in living cells and show that very closely related TFs may trigger significantly divergent outcomes at the same REs.Show less >
Show more >Transcription factor (TF) recruitment to chromatin is central to activation of transcription. TF-chromatin interactions are highly dynamic, which are evaluated by recovery half time (t1/2) in seconds, determined by fluorescence recovery experiments in living cells, and chromatin immunoprecipitation (ChIP) analysis, measured in minutes. These two states are related: the larger the t1/2, the longer the ChIP occupancy resulting in increased transcription. Here we present data showing that this relationship does not always hold. We found that histone deacetylase inhibitors (HDACis) significantly increased t1/2 of green fluorescent protein (GFP) fused androgen receptor (AR) on a tandem array of positive hormone response elements (HREs) in chromatin. This resulted in increased ChIP signal of GFP-AR. Unexpectedly, however, transcription was inhibited. In contrast, the GFP-fused glucocorticoid receptor (GR), acting through the same HREs, displayed a profile consistent with current models. We provide evidence that these differences are mediated, at least in part, by HDACs. Our results provide insight into TF action in living cells and show that very closely related TFs may trigger significantly divergent outcomes at the same REs.Show less >
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
CHU Lille
Inserm
Université de Lille
Inserm
Université de Lille
Research team(s) :
Therapeutic innovation targetting inflammation
Submission date :
2019-05-17T13:08:43Z