Divergent Binding and Transactivation by ...
Type de document :
Article dans une revue scientifique
DOI :
PMID :
URL permanente :
Titre :
Divergent Binding and Transactivation by Two Related Steroid Receptors at the Same Response Element
Auteur(s) :
Tesikova, Martina [Auteur]
Dezitter, Xavier [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Nenseth, Hatice Z. [Auteur]
Klokk, Tove I. [Auteur]
Mueller, Florian [Auteur]
Hager, Gordon L. [Auteur]
Saatcioglu, Fahri [Auteur]
Dezitter, Xavier [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Nenseth, Hatice Z. [Auteur]
Klokk, Tove I. [Auteur]
Mueller, Florian [Auteur]
Hager, Gordon L. [Auteur]
Saatcioglu, Fahri [Auteur]
Titre de la revue :
The Journal of biological chemistry
Nom court de la revue :
J. Biol. Chem.
Numéro :
291
Pagination :
11899-11910
Date de publication :
2016-05-27
ISSN :
0021-9258
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Transcription factor (TF) recruitment to chromatin is central to activation of transcription. TF-chromatin interactions are highly dynamic, which are evaluated by recovery half time (t1/2) in seconds, determined by ...
Lire la suite >Transcription factor (TF) recruitment to chromatin is central to activation of transcription. TF-chromatin interactions are highly dynamic, which are evaluated by recovery half time (t1/2) in seconds, determined by fluorescence recovery experiments in living cells, and chromatin immunoprecipitation (ChIP) analysis, measured in minutes. These two states are related: the larger the t1/2, the longer the ChIP occupancy resulting in increased transcription. Here we present data showing that this relationship does not always hold. We found that histone deacetylase inhibitors (HDACis) significantly increased t1/2 of green fluorescent protein (GFP) fused androgen receptor (AR) on a tandem array of positive hormone response elements (HREs) in chromatin. This resulted in increased ChIP signal of GFP-AR. Unexpectedly, however, transcription was inhibited. In contrast, the GFP-fused glucocorticoid receptor (GR), acting through the same HREs, displayed a profile consistent with current models. We provide evidence that these differences are mediated, at least in part, by HDACs. Our results provide insight into TF action in living cells and show that very closely related TFs may trigger significantly divergent outcomes at the same REs.Lire moins >
Lire la suite >Transcription factor (TF) recruitment to chromatin is central to activation of transcription. TF-chromatin interactions are highly dynamic, which are evaluated by recovery half time (t1/2) in seconds, determined by fluorescence recovery experiments in living cells, and chromatin immunoprecipitation (ChIP) analysis, measured in minutes. These two states are related: the larger the t1/2, the longer the ChIP occupancy resulting in increased transcription. Here we present data showing that this relationship does not always hold. We found that histone deacetylase inhibitors (HDACis) significantly increased t1/2 of green fluorescent protein (GFP) fused androgen receptor (AR) on a tandem array of positive hormone response elements (HREs) in chromatin. This resulted in increased ChIP signal of GFP-AR. Unexpectedly, however, transcription was inhibited. In contrast, the GFP-fused glucocorticoid receptor (GR), acting through the same HREs, displayed a profile consistent with current models. We provide evidence that these differences are mediated, at least in part, by HDACs. Our results provide insight into TF action in living cells and show that very closely related TFs may trigger significantly divergent outcomes at the same REs.Lire moins >
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
CHU Lille
Inserm
Université de Lille
Inserm
Université de Lille
Équipe(s) de recherche :
Therapeutic innovation targetting inflammation
Date de dépôt :
2019-05-17T13:08:43Z