Title :

Influence of the surface charge of PLGA nanoparticles on their in vitro genotoxicity, cytotoxicity, ROS production and endocytosis

Author(s) :

Platel, Anne [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Carpentier, Rodolphe [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Becart, Elodie [Auteur]
Institut Pasteur de Lille
Mordacq, Gwendoline [Auteur]
Institut Pasteur de Lille
Betbeder, Didier [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Nesslany, Fabrice [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]

Journal title :

Journal of applied toxicology

Abbreviated title :

J. Appl. Toxicol.

Volume number :

36

Pages :

434-444

Publisher :

Wiley

Publication date :

2016-03-01

ISSN :

0260-437X

English keyword(s) :

ROS
TK6 human lymphoblastoid cells
L5178Y mouse lymphoma cells
16HBE14o-human bronchial epithelial cells
genotoxicity
nanoparticles of PLGA
cytotoxicity
endocytosis

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

With the ongoing commercialization of nanotechnology products, human exposure to nanoparticles (NPs) is set to increase dramatically and an evaluation of their potential adverse effects is essential. Surface charge, among ...
Show more >With the ongoing commercialization of nanotechnology products, human exposure to nanoparticles (NPs) is set to increase dramatically and an evaluation of their potential adverse effects is essential. Surface charge, among other physico-chemicals parameters, is a key criterion that should be considered when using a definition for nanomaterials in a regulatory context. It has recently been recognized as an important factor in determining the toxicity of NPs; however, a complete understanding of the mechanisms involved is still lacking. In this context, the aim of the present study was to investigate the influence of the surface charge modification of NPs on in vitro toxicity assays. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles bearing different surface charges, positive(+), neutral(n) or negative(-), were synthesized. In vitro genotoxicity assays (micronucleus and comet assays) coupled with an assessment of cytotoxicity, were performed in different cell lines (L5178Y mouse lymphoma cells, TK6 human B-lymphoblastoid cells and 16HBE14o- human bronchial epithelial cells). Reactive oxygen species (ROS) production and endocytosis studies were also performed. Our results showed that PLGA(+) NPs were cytotoxic. They are endocytosed by the clathrin pathway and induced ROS in the three cell lines. They led to chromosomal aberrations without primary DNA damage in 16HBE14o- cells, suggesting that aneuploidy may be considered as an important biomarker when assessing the genotoxic potential of NPs. Moreover, 16HBE14o- cells seem to be more suitable for the in vitro screening of inhaled NPs than the regulatory L5178Y and TK6 cells.Show less >

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Inserm
Université de Lille
Institut Pasteur de Lille

Collections :

• IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Research team(s) :

Therapeutic innovation targetting inflammation

Submission date :

2019-05-17T13:14:37Z
2022-09-07T08:46:18Z
2024-02-15T10:12:36Z