Urinary PAH Metabolites as Biomarkers of Exposure in Aquatic Environments

Fillmann, Gilberto; Watson, Giles; Howsam, Michael; Francioni, Eleine; Depledge, Michael; Readman, James

Document type :

Article dans une revue scientifique: Article original

DOI :

10.1021/es0350839

Title :

Urinary PAH Metabolites as Biomarkers of Exposure in Aquatic Environments

Author(s) :

Fillmann, Gilberto [Auteur]
Watson, Giles [Auteur]
Plymouth Marine Laboratory [PML]
Howsam, Michael [Auteur]

Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 [RID-AGE]
Francioni, Eleine [Auteur]
Depledge, Michael [Auteur]
University of Exeter Medical School
Readman, James [Auteur]
Plymouth University

Journal title :

Environmental Science and Technology

Pages :

2649-2656

Publisher :

American Chemical Society

Publication date :

2004-03-30

ISSN :

0013-936X

English abstract : [en]

While immunoassays have been extensively applied to evaluate environmental contamination, to date they have rarely been used for the analysis of biological fluids outside of human medicine. These media are important because ...
Show more >While immunoassays have been extensively applied to evaluate environmental contamination, to date they have rarely been used for the analysis of biological fluids outside of human medicine. These media are important because pollutants such as polycyclic aromatic hydrocarbons (PAHs) and their metabolites become concentrated in tissues, body fluids, and excreta, thereby offering a measure of exposure to biologically available contaminants. Such analyses also provide a nondestructive tool for monitoring exposure. Crabs (Carcinus maenas) were exposed to phenanthrene and pyrene (separately) at concentrations ranging from 0 to 200 μg L-1. After 48 h, urine samples were taken and analyzed by immunoassay and UV-fluorescence spectrophotometry. Urinary levels (calibrated against hydroxylated metabolites) proved to be dose dependent for both compounds, and good agreement was demonstrated between the immunoassay and the fluorescence techniques. The cross reactivity of the immunochemical technique (ELISA) for pyrene and hydroxy-metabolites was lower than for phenanthrene. HPLC analyses demonstrated that urine from the crabs exposed to pyrene contained mainly conjugate PAH metabolites whose concentrations (the sum of the four main pyrene metabolites/conjugates) showed very good agreement with the ELISA (r2 > 0.94) and fluorescence (r2 > 0.91) data. Environmental samples were also analyzed by ELISA and UV-fluorescence, and both techniques detected PAH (mainly petrogenic) contamination in the urine samples from a polluted harbor. These data demonstrate the potential of urine analyses by ELISA and UV-fluorescence to measure exposure of crabs to PAH.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

Facteurs de risque et déterminants moléculaires des maladies liées au vieillissement (RID-AGE) - U1167

Source :

Harvested from HAL

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