Title :

A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening.

Author(s) :

Palmieri-Thiers, Cynthia [Auteur]
Canaan, Stéphane [Auteur]
Brunini, Virginie [Auteur]
Lorenzi, Vannina [Auteur]
Tomi, Félix [Auteur]
Desseyn, Jean-Luc [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Garscha, Ulrike [Auteur]
Oliw, Ernst H [Auteur]
Berti, Liliane [Auteur]
Maury, Jacques [Auteur]

Journal title :

Biochimica et biophysica acta

Abbreviated title :

Biochim Biophys Acta

Volume number :

1791

Pages :

339-46

Publication date :

2009-05-01

ISSN :

0006-3002

English keyword(s) :

Amino Acid Sequence
Base Sequence
Blotting
Southern
Chromatography
High Pressure Liquid
DNA
Complementary
Electrophoresis
Polyacrylamide Gel
Fruit
Gene Expression Regulation
Plant
Kinetics
Lipoxygenase
Magnetic Resonance Spectroscopy
Mass Spectrometry
Molecular Sequence Data
Olea
Polymorphism
Genetic
Recombinant Proteins
Substrate Specificity

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Plant lipoxygenases (LOXs) are a class of widespread dioxygenases catalysing the hydroperoxidation of polyunsaturated fatty acids. Although multiple isoforms of LOX have been detected in a wide range of plants, their ...
Show more >Plant lipoxygenases (LOXs) are a class of widespread dioxygenases catalysing the hydroperoxidation of polyunsaturated fatty acids. Although multiple isoforms of LOX have been detected in a wide range of plants, their physiological roles remain to be clarified. With the aim to clarify the occurrence of LOXs in olives and their contribution to the elaboration of the olive oil aroma, we cloned and characterized the first cDNA of the LOX isoform which is expressed during olive development. The open reading frame encodes a polypeptide of 864 amino acids. This olive LOX is a type-1 LOX which shows a high degree of identity at the peptide level towards hazelnut (77.3%), tobacco (76.3%) and almond (75.5%) LOXs. The recombinant enzyme shows a dual positional specificity, as it forms both 9- and 13-hydroperoxide of linoleic acid in a 2:1 ratio, and would be defined as 9/13-LOX. Although a LOX activity was detected throughout the olive development, the 9/13-LOX is mainly expressed at late developmental stages. Our data suggest that there are at least two Lox genes expressed in black olives, and that the 9/13-LOX is associated with the ripening and senescence processes. However, due to its dual positional specificity and its expression pattern, its contribution to the elaboration of the olive oil aroma might be considered.Show less >

Language :

Anglais

Audience :

Non spécifiée

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2019-07-09T13:56:15Z
2019-07-10T09:00:56Z