Title :

Dietary n-3 fatty acids have suppressive effects on mucin upregulation in mice infected with Pseudomonas aeruginosa.

Author(s) :

Tetaert, Daniel [Auteur]

Pierre, Maud [Auteur]
Demeyer, Dominique [Auteur]
Husson, Marie-Odile [Auteur]
Beghin, Laurent [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Galabert, Claude [Auteur]
gottrand, frederic [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Beermann, Christopher [Auteur]

Journal title :

Respiratory research

Abbreviated title :

Respir Res

Volume number :

8

Pages :

39

Publication date :

2007-06-05

ISSN :

1465-993X

English keyword(s) :

Administration
Oral
Animals
Fatty Acids
Omega-3
Lung
Male
Mice
Mice
Inbred C57BL
Mucins
Pseudomonas Infections
Treatment Outcome
Up-Regulation

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Mucin hypersecretion and mucus plugging in the airways are characteristic features of chronic respiratory diseases like cystic fibrosis (CF) and contribute to morbidity and mortality. In CF, Pseudomonas aeruginosa ...
Show more >Mucin hypersecretion and mucus plugging in the airways are characteristic features of chronic respiratory diseases like cystic fibrosis (CF) and contribute to morbidity and mortality. In CF, Pseudomonas aeruginosa superinfections in the lung exacerbate inflammation and alter mucus properties. There is increasing evidence that n-3 polyunsaturated fatty acids (PUFAs) exhibit anti-inflammatory properties in many inflammatory diseases while n-6 PUFA arachidonic acid (AA) favors inflammatory mediators such as eicosanoids prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) that may enhance inflammatory reactions. This suggests that n-3 PUFAs may have a protective effect against mucus over-production in airway diseases. Therefore, we hypothesized that n-3 PUFAs may downregulate mucins expression. We designed an absolute real-time PCR assay to assess the effect of a 5-week diet enriched either with n-3 or n-6 PUFAs on the expression of large mucins in the lungs of mice infected by P. aeruginosa. Dietary fatty acids did not influence mucin gene expression in healthy mice. Lung infection induced an increase of the secreted gel-forming mucin Muc5b and a decrease of the membrane bound mucin Muc4. These deregulations are modulated by dietary fatty acids with a suppressive effect of n-3 PUFAs on mucin (increase of Muc5b from 19-fold up to 3.6 x 10(5)-fold for the n-3 PUFAs treated group and the control groups, respectively, 4 days post-infection and decrease of Muc4 from 15-fold up to 3.2 x 10(4)-fold for the control and the n-3 PUFAs treated groups, respectively, 4 days post-infection). Our data suggest that n-3 PUFAs enriched diet represents an inexpensive strategy to prevent or treat mucin overproduction in pulmonary bacterial colonization.Show less >

Language :

Anglais

Audience :

Non spécifiée

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2019-07-09T14:06:06Z
2019-07-10T07:13:40Z