Title :

Tailor-made purified human platelet lysate concentrated in neurotrophins for treatment of parkinson's disease

Author(s) :

Chou, Ming-Li [Auteur]
Wu, Joe-Wei [Auteur]
Gouel, Flore [Auteur]
Jonneaux, Aurelie [Auteur]
Timmerman, Kelly [Auteur]
Troubles cognitifs dégénératifs et vasculaires - U1171
Troubles cognitifs dégénératifs et vasculaires - U 1171 [TCDV]
Troubles cognitifs dégénératifs et vasculaires - U 1171 [TCDV]
Renn, Ting-Yi [Auteur]
Laloux, Charlotte [Auteur]
Chang, Hung-Ming [Auteur]
Lin, Liang-Tzung [Auteur]
Devedjian, Jean-Christophe [Auteur]
DEVOS, DAVID [Auteur]
Troubles cognitifs dégénératifs et vasculaires - U1171
Troubles cognitifs dégénératifs et vasculaires - U 1171 [TCDV]
Troubles cognitifs dégénératifs et vasculaires - U 1171 [TCDV]
Burnouf, Thierry [Auteur]

Journal title :

Biomaterials

Abbreviated title :

Biomaterials

Volume number :

142

Pages :

77-89

Publication date :

2017-10-01

ISSN :

0142-9612

English keyword(s) :

Neuroprotection
Platelet lysate
Growth factors
Neurotrophins
MPTP
Parkinson

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Human platelet lysates (PLs), which contain multiple neurotrophins, have been proposed for treating neurodegenerative disorders, including Parkinson's disease (PD). However, current PLs suspended in plasma have high protein ...
Show more >Human platelet lysates (PLs), which contain multiple neurotrophins, have been proposed for treating neurodegenerative disorders, including Parkinson's disease (PD). However, current PLs suspended in plasma have high protein content and contain fibrinogen/fibrin and, following activation, also proteolytic and thrombogenic enzymes. Upon brain administration, such PLs may saturate the cerebrospinal fluid and exert neurotoxicity. We assessed whether purified PLs, concentrated in neurotrophins, protected dopaminergic neurons in PD models. Platelet concentrates were collected by apheresis and centrifuged to eliminate plasma and recover the platelets. Platelets were lysed by freeze-thaw cycles, and the 10-fold concentrated platelet pellet lysates (PPLs) were heat-treated (at 56 °C for 30 min). The heat-treated PPLs were low in total proteins, depleted in both plasma and platelet fibrinogen, and devoid of thrombogenic and proteolytic activities. They exerted very high neuroprotective activity when non-oncogenic, Lund human mesencephalic (LUHMES) cells that had differentiated into dopaminergic neurons were exposed to the MPP+Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
CNRS
Inserm
Université de Lille

Collections :

• Lille Neurosciences & Cognition (LilNCog) - U 1172

Research team(s) :

Troubles cognitifs dégénératifs et vasculaires

Submission date :

2019-11-27T13:37:34Z