Surface Plasmon Resonance (SPR) for the ...
Type de document :
Article dans une revue scientifique
DOI :
PMID :
URL permanente :
Titre :
Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion
Auteur(s) :
Zagorodko, Oleksandr [Auteur]
Bouckaert, Julie [Auteur]
Dumych, Tetiana [Auteur]
Bilyy, Rostyslav [Auteur]
Larroulet, Iban [Auteur]
Yanguas Serrano, Aritz [Auteur]
Alvarez Dorta, Dimitri [Auteur]
Gouin, Sébastien G. [Auteur]
Dima, Stefan-Ovidiu [Auteur]
Oancea, Florin [Auteur]
Boukherroub, Rabah [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Szunerits, Sabine [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Bouckaert, Julie [Auteur]
Dumych, Tetiana [Auteur]
Bilyy, Rostyslav [Auteur]
Larroulet, Iban [Auteur]
Yanguas Serrano, Aritz [Auteur]
Alvarez Dorta, Dimitri [Auteur]
Gouin, Sébastien G. [Auteur]
Dima, Stefan-Ovidiu [Auteur]
Oancea, Florin [Auteur]
Boukherroub, Rabah [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Szunerits, Sabine [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Titre de la revue :
Biosensors
Nom court de la revue :
Biosensors (Basel)
Numéro :
5
Pagination :
276-287
Date de publication :
2015-05-26
ISSN :
2079-6374
Mot(s)-clé(s) en anglais :
Bacterial Adhesion
carbohydrates
shear force enhancement
Surface Plasmon Resonance
Gold
Biomechanical Phenomena
Escherichia coli
Escherichia coli (E. coli)
Biosensing Techniques
Mannose
surface plasmon resonance (SPR)
flow rate
carbohydrates
shear force enhancement
Surface Plasmon Resonance
Gold
Biomechanical Phenomena
Escherichia coli
Escherichia coli (E. coli)
Biosensing Techniques
Mannose
surface plasmon resonance (SPR)
flow rate
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels ...
Lire la suite >The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.Lire moins >
Lire la suite >The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.Lire moins >
Langue :
Anglais
Établissement(s) :
ISEN
Univ. Valenciennes
CNRS
Institut Catholique Lille
Centrale Lille
Université de Lille
Univ. Valenciennes
CNRS
Institut Catholique Lille
Centrale Lille
Université de Lille
Collections :
Équipe(s) de recherche :
Computational Molecular Systems Biology
Date de dépôt :
2020-02-12T15:11:18Z