Title :

Structurally Flexible Triethanolamine Core PAMAM Dendrimers Are Effective Nanovectors for DNA Transfection in Vitro and in Vivo to the Mouse Thymus

Author(s) :

Liu, Xiaoxuan [Auteur]
Wu, Jiangyu [Auteur]
Yammine, Miriam [Auteur]
Technologies avancées pour le génôme et la clinique [TAGC]
Zhou, Jiehua [Auteur]
Posocco, Paola [Auteur]
Viel, Stéphane [Auteur]
Institut de Chimie Radicalaire [ICR]
Liu, Cheng [Auteur]
Ziarelli, Fabio [Auteur]
Fermeglia, Maurizio [Auteur]
Pricl, Sabrina [Auteur]
Victorero, Genevieve [Auteur]
Technologies avancées pour le génôme et la clinique [TAGC]
Nguyen, Catherine [Auteur]
Technologies avancées pour le génôme et la clinique [TAGC]
Erbacher, Patrick [Auteur]
Behr, Jean-Paul [Auteur]
Institut Gilbert-Laustriat : Biomolécules, Biotechnologie, Innovation Thérapeutique
Laboratoire de chimie bioorganique [LCB]
Peng, Ling [Auteur]
Laboratoire d’Électrotechnique et d’Électronique de Puissance - ULR 2697 [L2EP]

Journal title :

Bioconjugate Chemistry

Volume number :

22

Pages :

2461--2473

Publisher :

American Chemical Society

Publication date :

2011-12

ISSN :

1043-1802

English keyword(s) :

Rodent models
Gene delivery
Genetics
Dendrons
Molecules

HAL domain(s) :

Chimie

English abstract : [en]

With the aim of developing dendrimer nanovectors with a precisely controlled architecture and flexible structure for DNA transfection, we designed PAMAM dendrimers bearing a triethanolamine (TEA) core, with branching units ...
Show more >With the aim of developing dendrimer nanovectors with a precisely controlled architecture and flexible structure for DNA transfection, we designed PAMAM dendrimers bearing a triethanolamine (TEA) core, with branching units pointing away from the center to create void spaces, reduce steric congestion, and increase water accessibility for the benefit of DNA delivery. These dendrimers are shown to form stable nanoparticles with DNA, promote cell uptake mainly via macropinocytosis, and act as effective nanovectors for DNA transfection in vitro on epithelial and fibroblast cells and, most importantly, in vivo in the mouse thymus, an exceedingly challenging organ for immune gene therapy. Collectively, these results validate our rational design approach of structurally flexible dendrimers with a chemically defined structure as effective nanovectors for gene delivery, and demonstrate the potential of these dendrimers in intrathymus gene delivery for future applications in immune gene therapy.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Comment :

https://hal.archives-ouvertes.fr/hal-01460316

Collections :

• Laboratoire d'Électrotechnique et d'Électronique de Puissance (L2EP) - ULR 2697

Submission date :

2020-05-15T13:48:40Z
2022-02-16T09:34:39Z