Title :

Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages

Author(s) :

Chua, Adeline [Auteur]
Ong, Jessica Jie Ying [Auteur]
Malleret, Benoit [Auteur]
Institut des Maladies Emergentes et des Thérapies Innovantes [IMETI]
Suwanarusk, Rossarin [Auteur]
Kosaisavee, Varakorn [Auteur]
Zeeman, Anne-Marie [Auteur]
Cooper, Caitlin [Auteur]
Tan, Kevin [Auteur]
Zhang, Rou [Auteur]
Tan, Bee [Auteur]
Abas, Siti [Auteur]
Yip, Andy [Auteur]
Elliot, Anne [Auteur]
Joyner, Chester [Auteur]
Cho, Jee [Auteur]
Breyer, Kate [Auteur]
Baran, Szczepan [Auteur]
Lange, Amber [Auteur]
Maher, Steven [Auteur]
Nosten, François [Auteur]
Mahidol Oxford Tropical Medicine Research Unit
Bodenreider, Christophe [Auteur]
Yeung, Bryan [Auteur]
Mazier, Dominique [Auteur]
Centre d'Immunologie et de Maladies Infectieuses [CIMI]
Galinski, Mary [Auteur]
Dereuddre-Bosquet, Nathalie [Auteur]
Immunologie des Maladies Virales et Autoimmunes [IMVA - U1184]
Le Grand, Roger [Auteur]
Immunologie des Maladies Virales et Autoimmunes [IMVA - U1184]
Kocken, Clemens [Auteur]
Biomedical Primate Research Centre [Rijswijk] [BPRC]
Renia, Laurent [Auteur]
Institut Cochin [UMR_S567 / UMR 8104]
Kyle, Dennis [Auteur]
Diagana, Thierry [Auteur]
Novartis Institute for Tropical Diseases [NITD]
Snounou, Georges [Auteur]
Immunologie des Maladies Virales et Autoimmunes [IMVA - U1184]
Russell, Bruce [Auteur]
Bifani, Pablo [Auteur]

Journal title :

Nature Communications

Publisher :

Nature Publishing Group

Publication date :

2019-12

ISSN :

2041-1723

English keyword(s) :

Plasmodium cynomolgi
in vitro culture
Drug discovery

HAL domain(s) :

Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Parasitologie
Sciences du Vivant [q-bio]/Biotechnologies

English abstract : [en]

The ability to culture pathogenic organisms substantially enhances the quest for fundamental knowledge and the development of vaccines and drugs. Thus, the elaboration of a protocol for the in vitro cultivation of the ...
Show more >The ability to culture pathogenic organisms substantially enhances the quest for fundamental knowledge and the development of vaccines and drugs. Thus, the elaboration of a protocol for the in vitro cultivation of the erythrocytic stages of Plasmodium falciparum revolutionized research on this important parasite. However, for P. vivax, the most widely distributed and difficult to treat malaria parasite, a strict preference for reticulocytes thwarts efforts to maintain it in vitro. Cultivation of P. cynomolgi, a macaque-infecting species phylogenetically close to P. vivax, was briefly reported in the early 1980s, but not pursued further. Here, we define the conditions under which P. cynomolgi can be adapted to long term in vitro culture to yield parasites that share many of the morphological and phenotypic features of P. vivax. Wefurther validate the potential of this culture system for high-throughput screening to prime and accelerate anti-P. vivax drug discovery efforts.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

ANR Project :

Recherches sur l'Hypnozoïte du Paludisme

Collections :

• Centre de Recherche en Informatique, Signal et Automatique de Lille (CRIStAL) - UMR 9189

Source :

Harvested from HAL