Inoculation Pneumonia Caused by Coagulase ...
Document type :
Compte-rendu et recension critique d'ouvrage
DOI :
PMID :
Title :
Inoculation Pneumonia Caused by Coagulase Negative Staphylococcus
Author(s) :
Shi, Meng-Meng [Auteur]
Shanghai Jiao Tong University School of Medicine
Monsel, Antoine [Auteur]
Immunologie - Immunopathologie - Immunothérapie [CHU Pitié Salpêtrière] [I3]
CHU Pitié-Salpêtrière [AP-HP]
Rouby, Jean-Jacques [Auteur]
CHU Pitié-Salpêtrière [AP-HP]
Xu, Yan-Ping [Auteur]
Shanghai Jiao Tong University School of Medicine
Zhu, Ying-Gang [Auteur]
Fudan University [Shanghai]
Qu, Jie-Ming [Auteur]
Shanghai Jiao Tong University School of Medicine
Shanghai Jiao Tong University School of Medicine
Monsel, Antoine [Auteur]
Immunologie - Immunopathologie - Immunothérapie [CHU Pitié Salpêtrière] [I3]
CHU Pitié-Salpêtrière [AP-HP]
Rouby, Jean-Jacques [Auteur]
CHU Pitié-Salpêtrière [AP-HP]
Xu, Yan-Ping [Auteur]
Shanghai Jiao Tong University School of Medicine
Zhu, Ying-Gang [Auteur]
Fudan University [Shanghai]
Qu, Jie-Ming [Auteur]
Shanghai Jiao Tong University School of Medicine
Journal title :
Frontiers in Microbiology
Pages :
2198
Publisher :
Frontiers Media
Publication date :
2019
ISSN :
1664-302X
English keyword(s) :
Staphylococcus haemolyticus
coagulase-negative staphylococci
inoculation pneumonia
macrophage inflammatory protein-2
tumor necrosis factor-α
coagulase-negative staphylococci
inoculation pneumonia
macrophage inflammatory protein-2
tumor necrosis factor-α
HAL domain(s) :
Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Maladies infectieuses
Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Pneumologie et système respiratoire
Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Pneumologie et système respiratoire
English abstract : [en]
Rationale: Although frequently retrieved in tracheal secretions of critically ill patients on mechanical ventilation, the existence of pneumonia caused by coagulase-negative staphylococci (CoNS) remains controversial.Objective: ...
Show more >Rationale: Although frequently retrieved in tracheal secretions of critically ill patients on mechanical ventilation, the existence of pneumonia caused by coagulase-negative staphylococci (CoNS) remains controversial.Objective: To assess whether Staphylococcus haemolyticus (S. haemolyticus) inoculated in mice's trachea can infect normal lung parenchyma, increasing concentrations of S. haemolyticus were intratracheally administered in 221 immunocompetent mice.Methods: Each animal received intratracheally phosphate-buffered saline (PBS) (n = 43) or live (n = 141) or inactivated (n = 37) S. haemolyticus at increasing load: 1.0 × 106, 1.0 × 107, and 1.0 × 108 colony forming units (CFU). Forty-three animals were sacrificed at 12 h and 178 were sacrificed at 36 h; 64 served for post-mortem lung histology, 157 served for pre-mortem bronchoalveolar lavage (BAL) analysis, and 42 served for post-mortem quantitative bacteriology of lung tissue. The distribution of biofilm-associated genes was investigated in the S. haemolyticus strain used in our in vivo experiment as well as among 19 other clinical S. haemolyticus strains collected from hospitals or nursing houses.Measurements and Main Results: Intratracheal inoculation of 1.0 × 108 CFU live S. haemolyticus caused macroscopic and histological confluent pneumonia with significant increase in BAL white cell count, tumor necrosis factor-α (TNF-α), and macrophage inflammatory protein (MIP)-2. At 12 h, high concentrations of S. haemolyticus were identified in BAL. At 36 h, lung injury and BAL inflammation were less severe than at 12 h and moderate concentrations of species belonging to the oropharyngeal flora were identified in lung tissue. The inoculation of 1.0 × 106 and 1.0 × 107 CFU live S. haemolyticus caused histologic interstitial pneumonia and moderate BAL inflammation. Similar results were observed after inoculation of inactivated S. haemolyticus. Moreover, biofilm formation was a common phenotype in S. haemolyticus isolates. The low prevalence of the ica operon in our clinical S. haemolyticus strain collection indicated icaA and icaD independent-biofilm formation.Conclusion: In immunocompetent spontaneously breathing mice, inoculation of S. haemolyticus causes concentration-dependent lung infection that spontaneously recovers over time. icaA and icaD independent biofilm formation is a common phenotype in S. haemolyticus isolates.Show less >
Show more >Rationale: Although frequently retrieved in tracheal secretions of critically ill patients on mechanical ventilation, the existence of pneumonia caused by coagulase-negative staphylococci (CoNS) remains controversial.Objective: To assess whether Staphylococcus haemolyticus (S. haemolyticus) inoculated in mice's trachea can infect normal lung parenchyma, increasing concentrations of S. haemolyticus were intratracheally administered in 221 immunocompetent mice.Methods: Each animal received intratracheally phosphate-buffered saline (PBS) (n = 43) or live (n = 141) or inactivated (n = 37) S. haemolyticus at increasing load: 1.0 × 106, 1.0 × 107, and 1.0 × 108 colony forming units (CFU). Forty-three animals were sacrificed at 12 h and 178 were sacrificed at 36 h; 64 served for post-mortem lung histology, 157 served for pre-mortem bronchoalveolar lavage (BAL) analysis, and 42 served for post-mortem quantitative bacteriology of lung tissue. The distribution of biofilm-associated genes was investigated in the S. haemolyticus strain used in our in vivo experiment as well as among 19 other clinical S. haemolyticus strains collected from hospitals or nursing houses.Measurements and Main Results: Intratracheal inoculation of 1.0 × 108 CFU live S. haemolyticus caused macroscopic and histological confluent pneumonia with significant increase in BAL white cell count, tumor necrosis factor-α (TNF-α), and macrophage inflammatory protein (MIP)-2. At 12 h, high concentrations of S. haemolyticus were identified in BAL. At 36 h, lung injury and BAL inflammation were less severe than at 12 h and moderate concentrations of species belonging to the oropharyngeal flora were identified in lung tissue. The inoculation of 1.0 × 106 and 1.0 × 107 CFU live S. haemolyticus caused histologic interstitial pneumonia and moderate BAL inflammation. Similar results were observed after inoculation of inactivated S. haemolyticus. Moreover, biofilm formation was a common phenotype in S. haemolyticus isolates. The low prevalence of the ica operon in our clinical S. haemolyticus strain collection indicated icaA and icaD independent-biofilm formation.Conclusion: In immunocompetent spontaneously breathing mice, inoculation of S. haemolyticus causes concentration-dependent lung infection that spontaneously recovers over time. icaA and icaD independent biofilm formation is a common phenotype in S. haemolyticus isolates.Show less >
Language :
Anglais
Popular science :
Non
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