Title :

High-Throughput Immunogenetics for Clinical and Research Applications in Immunohematology: Potential and Challenges.

Author(s) :

Langerak, Anton [Auteur]
Erasmus University Medical Center [Rotterdam] [Erasmus MC]
Brüggemann, Monika [Auteur]
Christian-Albrechts-Universität zu Kiel = Christian-Albrechts University of Kiel = Université Christian-Albrechts de Kiel [CAU]
Davi, Frederic [Auteur]
CHU Pitié-Salpêtrière [AP-HP]
Darzentas, Nikos [Auteur]
Central European Institute of Technology [Brno] [CEITEC MU]
van Dongen, Jacques [Auteur]
Erasmus University Medical Center [Rotterdam] [Erasmus MC]
Gonzalez, David [Auteur]
Queen's University [Belfast] [QUB]
Cazzaniga, Gianni [Auteur]
Centro Ricerca Tettamanti
Giudicelli, Véronique [Auteur]
Institut de génétique humaine [IGH]
Lefranc, Marie-Paule [Auteur]
Institut de génétique humaine [IGH]
Giraud, Mathieu [Auteur]
Centre de Recherche en Informatique, Signal et Automatique de Lille - UMR 9189 [CRIStAL]
Bioinformatics and Sequence Analysis [BONSAI]
Macintyre, Elizabeth [Auteur]
Hôpital Necker - Enfants Malades [AP-HP]
Hummel, Michael [Auteur]
Charité - UniversitätsMedizin = Berlin University Medicine
Pott, Christiane [Auteur]
Christian-Albrechts-Universität zu Kiel = Christian-Albrechts University of Kiel = Université Christian-Albrechts de Kiel [CAU]
Groenen, Patricia Jta [Auteur]
Radboud University Medical Center [Nijmegen] [RadboudUMC]
Stamatopoulos, Kostas [Auteur]
Centre for Research and Technology Hellas [CERTH]
Karolinska Institutet [Stockholm]

Journal title :

Journal of Immunology

Pages :

3765-3774

Publisher :

Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists

Publication date :

2017-05-15

ISSN :

0022-1767

HAL domain(s) :

Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Hématologie
Sciences du Vivant [q-bio]/Immunologie

English abstract : [en]

Analysis and interpretation of Ig and TCR gene rearrangements in the conventional, low-throughput way have their limitations in terms of resolution, coverage, and biases. With the advent of high-throughput, next-generation ...
Show more >Analysis and interpretation of Ig and TCR gene rearrangements in the conventional, low-throughput way have their limitations in terms of resolution, coverage, and biases. With the advent of high-throughput, next-generation sequencing (NGS) technologies, a deeper analysis of Ig and/or TCR (IG/TR) gene rearrangements is now within reach, which impacts on all main applications of IG/TR immunogenetic analysis. To bridge the generation gap from low- to high-throughput analysis, the EuroClonality-NGS Consortium has been formed, with the main objectives to develop, standardize, and validate the entire workflow of IG/TR NGS assays for 1) clonality assessment, 2) minimal residual disease detection, and 3) repertoire analysis. This concerns the preanalytical (sample preparation, target choice), analytical (amplification, NGS), and postanalytical (immunoinformatics) phases. Here we critically discuss pitfalls and challenges of IG/TR NGS methodology and its applications in hemato-oncology and immunology.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre de Recherche en Informatique, Signal et Automatique de Lille (CRIStAL) - UMR 9189

Source :

Harvested from HAL