Title :

A novel RNA-binding peptide regulates the establishment of the Medicago truncatula-Sinorhizobium meliloti nitrogen-fixing symbiosis.

Author(s) :

Laporte, Philippe [Auteur]
Laboratoire d'Informatique Fondamentale de Lille [LIFL]
Nouveaux Outils pour La Coopération et l'Education [NOCE]
Satiat-Jeunemaître, Béatrice [Auteur]
Institut des sciences du végétal [ISV]
Velasco, Isabel [Auteur]
Csorba, Tibor [Auteur]
van de Velde, Willem [Auteur]
Center for Plant Systems Biology [PSB Center]
Institut des sciences du végétal [ISV]
Campalans, Anna [Auteur]
Laboratoire de Recherche sur l'Instabilité Génétique [LRIG]
Burgyan, Joszef [Auteur]
Arevalo-Rodriguez, Miguel [Auteur]
Crespi, Martin [Auteur]
Institut des sciences du végétal [ISV]

Journal title :

The Plant Journal

Pages :

24-38

Publisher :

Wiley

Publication date :

2010-04-01

ISSN :

0960-7412

HAL domain(s) :

Sciences du Vivant [q-bio]/Biologie végétale

English abstract : [en]

Plants use a variety of small peptides for cell to cell communication during growth and development. Leguminous plants are characterized by their ability to develop nitrogen-fixing nodules via an interaction with symbiotic ...
Show more >Plants use a variety of small peptides for cell to cell communication during growth and development. Leguminous plants are characterized by their ability to develop nitrogen-fixing nodules via an interaction with symbiotic bacteria. During nodule organogenesis, several so-called nodulin genes are induced, including large families that encode small peptides. Using a three-hybrid approach in yeast cells, we identified two new small nodulins, MtSNARP1 and MtSNARP2 (for small nodulin acidic RNA-binding protein), which interact with the RNA of MtENOD40, an early induced nodulin gene showing conserved RNA secondary structures. The SNARPs are acidic peptides showing single-stranded RNA-binding activity in vitro and are encoded by a small gene family in Medicago truncatula. These peptides exhibit two new conserved motifs and a putative signal peptide that redirects a GFP fusion to the endoplasmic reticulum both in protoplasts and during symbiosis, suggesting they are secreted. MtSNARP2 is expressed in the differentiating region of the nodule together with several early nodulin genes. MtSNARP2 RNA interference (RNAi) transgenic roots showed aberrant early senescent nodules where differentiated bacteroids degenerate rapidly. Hence, a functional symbiotic interaction may be regulated by secreted RNA-binding peptides.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre de Recherche en Informatique, Signal et Automatique de Lille (CRIStAL) - UMR 9189

Source :

Harvested from HAL