Title :

Characterization of two strains of <em>Anaplasma marginale</em> isolated from cattle in Rio de Janeiro, Brazil, after propagation in tick cell culture.

Author(s) :

Baêta, Bruna A. [Auteur]
Universidade Federal Rural do Rio de Janeiro [UFRRJ]
Ribeiro, Carla C.D.U [Auteur]
Universidade Federal Rural do Rio de Janeiro [UFRRJ]
Teixeira, Rafaella C. [Auteur]
Universidade Federal Rural do Rio de Janeiro [UFRRJ]
Cabezas-Cruz, Alejandro [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Passos, Lygia M.F. [Auteur]
Universidade Federal de Minas Gerais = Federal University of Minas Gerais [Belo Horizonte, Brazil] [UFMG]
Zweygarth, Erich [Auteur]
Fonseca, Adivaldo H. [Auteur correspondant]
Universidade Federal Rural do Rio de Janeiro [UFRRJ]

Journal title :

Ticks and Tick-borne Diseases

Pages :

141-145

Publisher :

Elsevier

Publication date :

2015

ISSN :

1877-959X

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

IDE8 tick cell cultures have been used for the isolation and propagation of several isolates of Anaplasma marginale. The genetic heterogeneity of A. marginale strains in cattle is diverse in endemic regions worldwide and ...
Show more >IDE8 tick cell cultures have been used for the isolation and propagation of several isolates of Anaplasma marginale. The genetic heterogeneity of A. marginale strains in cattle is diverse in endemic regions worldwide and the analyses of msp1˛ (major surface protein 1 alpha) gene sequences have allowed the identification of different strains. This study reports the isolation and propagation of two new isolates of A. marginale in IDE8 cells from blood of two cattle and their morphological and molecular characterization using light microscopy and the msp1˛ gene, respectively. Small colonies were observed in cytospin smears of each of the isolates 60 days after culture initiation. Based on msp1˛ sequence variation, the two isolates were found to be separate strains and were named AmRio1 and AmRio2. Analysis of msp1˛ microsatellite in both strains resulted in a single genotype, genotype E. The amino acid sequence of one MSP1 tandem repeat from the strain AmRio1 resulted in a new sequence (named 162) with one amino acid change. The results ofthese phylogenetic analyses demonstrated that A. marginale strains from Brazil and Argentina formed two large clusters of which one was less divergent that the other.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Comment :

Contribution: Sequence and phylogenetic analysis.

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL