Key experimental evidence of chromosomal ...
Type de document :
Compte-rendu et recension critique d'ouvrage
DOI :
PMID :
Titre :
Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria
Auteur(s) :
Boritsch, Eva [Auteur]
Pathogénomique mycobactérienne intégrée
Khanna, Varun [Auteur]
Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB
Pawlik, Alexandre [Auteur]
Pathogénomique mycobactérienne intégrée
Honoré, Nadine [Auteur]
Pathogénomique mycobactérienne intégrée
Navas, Victor [Auteur]
Biologie Cellulaire des Lymphocytes - Lymphocyte Cell Biology
Ma, Laurence [Auteur]
Génomique (Plate-Forme) - Genomics Platform
Bouchier, Christiane [Auteur]
Génomique (Plate-Forme) - Genomics Platform
Seemann, Torsten [Auteur]
Supply, Philip [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Stinear, Timothy [Auteur]
Brosch, Roland [Auteur correspondant]
Pathogénomique mycobactérienne intégrée
Pathogénomique mycobactérienne intégrée
Khanna, Varun [Auteur]
Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB
Pawlik, Alexandre [Auteur]
Pathogénomique mycobactérienne intégrée
Honoré, Nadine [Auteur]
Pathogénomique mycobactérienne intégrée
Navas, Victor [Auteur]
Biologie Cellulaire des Lymphocytes - Lymphocyte Cell Biology
Ma, Laurence [Auteur]
Génomique (Plate-Forme) - Genomics Platform
Bouchier, Christiane [Auteur]
Génomique (Plate-Forme) - Genomics Platform
Seemann, Torsten [Auteur]
Supply, Philip [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Stinear, Timothy [Auteur]
Brosch, Roland [Auteur correspondant]
Pathogénomique mycobactérienne intégrée
Titre de la revue :
Proceedings of the National Academy of Sciences of the United States of America
Pagination :
9876-9881
Éditeur :
National Academy of Sciences
Date de publication :
2016-08-30
ISSN :
0027-8424
Mot(s)-clé(s) en anglais :
DNA transfer
Mycobacterium canettii
evolution
recombination
tuberculosis
Mycobacterium canettii
evolution
recombination
tuberculosis
Discipline(s) HAL :
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
Sciences du Vivant [q-bio]/Biodiversité/Evolution [q-bio.PE]
Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Maladies infectieuses
Sciences du Vivant [q-bio]/Biodiversité/Evolution [q-bio.PE]
Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Maladies infectieuses
Résumé en anglais : [en]
Horizontal gene transfer (HGT) is a major driving force of bacterial diversification and evolution. For tuberculosis-causing mycobacteria, the impact of HGT in the emergence and distribution of dominant lineages remains a ...
Lire la suite >Horizontal gene transfer (HGT) is a major driving force of bacterial diversification and evolution. For tuberculosis-causing mycobacteria, the impact of HGT in the emergence and distribution of dominant lineages remains a matter of debate. Here, by using fluorescence-assisted mating assays and whole genome sequencing, we present unique experimental evidence of chromosomal DNA transfer between tubercle bacilli of the early-branching Mycobacterium canettii clade. We found that the obtained recombinants had received multiple donor-derived DNA fragments in the size range of 100 bp to 118 kbp, fragments large enough to contain whole operons. Although the transfer frequency between M. canettii strains was low and no transfer could be observed among classical Mycobacterium tuberculosis complex (MTBC) strains, our study provides the proof of concept for genetic exchange in tubercle bacilli. This outstanding, now experimentally validated phenomenon presumably played a key role in the early evolution of the MTBC toward pathogenicity. Moreover, our findings also provide important information for the risk evaluation of potential transfer of drug resistance and fitness mutations among clinically relevant mycobacterial strains.Lire moins >
Lire la suite >Horizontal gene transfer (HGT) is a major driving force of bacterial diversification and evolution. For tuberculosis-causing mycobacteria, the impact of HGT in the emergence and distribution of dominant lineages remains a matter of debate. Here, by using fluorescence-assisted mating assays and whole genome sequencing, we present unique experimental evidence of chromosomal DNA transfer between tubercle bacilli of the early-branching Mycobacterium canettii clade. We found that the obtained recombinants had received multiple donor-derived DNA fragments in the size range of 100 bp to 118 kbp, fragments large enough to contain whole operons. Although the transfer frequency between M. canettii strains was low and no transfer could be observed among classical Mycobacterium tuberculosis complex (MTBC) strains, our study provides the proof of concept for genetic exchange in tubercle bacilli. This outstanding, now experimentally validated phenomenon presumably played a key role in the early evolution of the MTBC toward pathogenicity. Moreover, our findings also provide important information for the risk evaluation of potential transfer of drug resistance and fitness mutations among clinically relevant mycobacterial strains.Lire moins >
Langue :
Anglais
Vulgarisation :
Non
Projet ANR :
Source :
Fichiers
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5024641/pdf
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