Title :

CXCR1/2 Antagonism Is Protective during Influenza and Post-Influenza Pneumococcal Infection

Author(s) :

Tavares, Luciana [Auteur]
Department of Biochemistry and Immunology, Institute of Biological Science
Garcia, Cristiana [Auteur]
Instituto Oswaldo Cruz = Oswaldo Cruz Institute [Rio de Janeiro] [IOC]
Machado, Marina [Auteur]
Departamento de Análises Clínicas e Toxicológicas [ACT]
Department of Biochemistry and Immunology, Institute of Biological Science
Queiroz-Junior, Celso [Auteur]
Departamento de Morfologia
Barthélémy, Adeline [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Trottein, Francois [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Siqueira, Marilda [Auteur]
Instituto Oswaldo Cruz = Oswaldo Cruz Institute [Rio de Janeiro] [IOC]
Brandolini, Laura [Auteur]
Allegretti, Marcello [Auteur]
Machado, Alexandre [Auteur]
Fiocruz Minas - René Rachou Research Center / Instituto René Rachou [Belo Horizonte, Brésil]
de Sousa, Lirlândia [Auteur]
Instituto Oswaldo Cruz = Oswaldo Cruz Institute [Rio de Janeiro] [IOC]
Teixeira, Mauro [Auteur correspondant]
Department of Biochemistry and Immunology, Institute of Biological Science

Journal title :

Frontiers in Immunology

Pages :

1799

Publisher :

Frontiers

Publication date :

2017-12-13

ISSN :

1664-3224

English keyword(s) :

CXCR1/2
immunomodulation
inflammation
influenza A
pneumococcus
secondary infection

HAL domain(s) :

Sciences du Vivant [q-bio]/Immunologie/Immunité innée

English abstract : [en]

RATIONALE:Influenza A infections are a leading cause of morbidity and mortality worldwide especially when associated with secondary pneumococcal infections. Inflammation is important to control pathogen proliferation but ...
Show more >RATIONALE:Influenza A infections are a leading cause of morbidity and mortality worldwide especially when associated with secondary pneumococcal infections. Inflammation is important to control pathogen proliferation but may also cause tissue injury and death. CXCR1/2 are chemokine receptors relevant for the recruitment of neutrophils. We investigated the role of CXCR1/2 during influenza, pneumococcal, and post-influenza pneumococcal infections.METHODS:Mice were infected with influenza A virus (IAV) or Streptococcus pneumoniae and then treated daily with the CXCR1/2 antagonist DF2162. To study secondary pneumococcal infection, mice were infected with a sublethal inoculum of IAV then infected with S. pneumoniae 14 days later. DF2162 was given in a therapeutic schedule from days 3 to 6 after influenza infection. Lethality, weight loss, inflammation, virus/bacteria counts, and lung injury were assessed.RESULTS:CXCL1 and CXCL2 were produced at high levels during IAV infection. DF2162 treatment decreased morbidity and this was associated with decreased infiltration of neutrophils in the lungs and reduced pulmonary damage and viral titers. During S. pneumoniae infection, DF2162 treatment decreased neutrophil recruitment, pulmonary damage, and lethality rates, without affecting bacteria burden. Therapeutic treatment with DF2162 during sublethal IAV infection reduced the morbidity associated with virus infection and also decreased the magnitude of inflammation, lung damage, and number of bacteria in the blood of mice subsequently infected with S. pneumoniae.CONCLUSION:Modulation of the inflammatory response by blocking CXCR1/2 improves disease outcome during respiratory influenza and pneumococcal infections, without compromising the ability of the murine host to deal with infection. Altogether, inhibition of CXCR1/2 may be a valid therapeutic strategy for treating lung infections caused by these pathogens, especially controlling secondary bacterial infection after influenza.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Comment :

Luciana P. Tavares and Cristiana C. Garcia have contributed equally to this work.

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL