Title :

East-Asian Helicobacter pylori strains synthesize heptan-deficient lipopolysaccharide

Author(s) :

Li, Hong [Auteur]
Marceau, Michael [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Yang, Tiandi [Auteur]
Department of Life Sciences
Liao, Tingting [Auteur]
Tang, Xiaoqiong [Auteur]
Hu, Renwei [Auteur]
Xie, Yan [Auteur]
Tang, Hong [Auteur correspondant]
Tay, Alfred [Auteur]
Shi, Ying [Auteur]
Shen, Yalin [Auteur]
Yang, Tiankuo [Auteur]
Pi, Xuenan [Auteur]
Lamichhane, Binit [Auteur]
Luo, Yong [Auteur]
Debowski, Aleksandra [Auteur]
Nilsson, Hans-Olof [Auteur]
Haslam, Stuart [Auteur]
Department of Life Sciences
Mulloy, Barbara [Auteur]
Department of Life Sciences
Dell, Anne [Auteur]
Department of Life Sciences
Stubbs, Keith [Auteur]
Marshall, Barry [Auteur]
Benghezal, Mohammed [Auteur correspondant]

Journal title :

PLoS Genetics

Pages :

e1008497

Publisher :

Public Library of Science

Publication date :

2019-11-20

ISSN :

1553-7390

HAL domain(s) :

Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Maladies infectieuses
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire

English abstract : [en]

The lipopolysaccharide O-antigen structure expressed by the European Helicobacter pylori model strain G27 encompasses a trisaccharide, an intervening glucan-heptan and distal Lewis antigens that promote immune escape. ...
Show more >The lipopolysaccharide O-antigen structure expressed by the European Helicobacter pylori model strain G27 encompasses a trisaccharide, an intervening glucan-heptan and distal Lewis antigens that promote immune escape. However, several gaps still remain in the corresponding biosynthetic pathway. Here, systematic mutagenesis of glycosyltransferase genes in G27 combined with lipopolysaccharide structural analysis, uncovered HP0102 as the trisaccharide fucosyltransferase, HP1283 as the heptan transferase, and HP1578 as the GlcNAc transferase that initiates the synthesis of Lewis antigens onto the heptan motif. Comparative genomic analysis of G27 lipopolysaccharide biosynthetic genes in strains of different ethnic origin revealed that East-Asian strains lack the HP1283/HP1578 genes but contain an additional copy of HP1105 and JHP0562. Further correlation of different lipopolysaccharide structures with corresponding gene contents led us to propose that the second copy of HP1105 and the JHP0562 may function as the GlcNAc and Gal transferase, respectively, to initiate synthesis of the Lewis antigen onto the Glc-Trio-Core in East-Asian strains lacking the HP1283/HP1578 genes. In view of the high gastric cancer rate in East Asia, the absence of the HP1283/HP1578 genes in East-Asian H. pylori strains warrants future studies addressing the role of the lipopolysaccharide heptan in pathogenesis.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL