Title :

The puzzling construction of the conidial outer layer of Aspergillus fumigatus

Author(s) :

Valsecchi, Isabel [Auteur]
Bioinformatique structurale - Structural Bioinformatics
Plateforme technologique de RMN biologique - Biological NMR Technological Platform
Aspergillus
Dupres, Vincent [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Michel, Jean-Philippe [Auteur]
Institut Galien Paris-Sud [IGPS]
Duchateau, Magalie [Auteur]
Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology [UTechS MSBio]
Matondo, Mariette [Auteur]
Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology [UTechS MSBio]
Chamilos, Georgios [Auteur]
University of Crete [Heraklion] [UOC]
Saveanu, Cosmin [Auteur]
Génétique des Interactions macromoléculaires / Genetics of Macromolecular Interactions
Guijarro, J. Iñaki [Auteur]
Bioinformatique structurale - Structural Bioinformatics
Plateforme technologique de RMN biologique - Biological NMR Technological Platform
Aimanianda, Vishukumar [Auteur]
Aspergillus
Lafont, Frank [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Latgé, Jean-Paul [Auteur]
Aspergillus
Beauvais, Anne [Auteur correspondant]
Aspergillus

Journal title :

Cellular Microbiology

Pages :

e12994

Publisher :

Wiley

Publication date :

2019-05

ISSN :

1462-5814

English keyword(s) :

Aspergillus
cell wall
chitin
galactosaminogalactan
glucan
hydrophobin
surface

HAL domain(s) :

Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Mycologie

English abstract : [en]

If the mycelium of Aspergillus fumigatus is very short-lived in the laboratory, conidia can survive for years. This survival capacity and extreme resistance to environmental insults is a major biological characteristic of ...
Show more >If the mycelium of Aspergillus fumigatus is very short-lived in the laboratory, conidia can survive for years. This survival capacity and extreme resistance to environmental insults is a major biological characteristic of this fungal species. Moreover, conidia, which easily reach the host alveola, are the infective propagules. Earlier studies have shown the role of some molecules of the outer conidial layer in protecting the fungus against the host defense. The outer layer of the conidial cell wall, directly in contact with the host cells, consists of α-(1,3)-glucan, melanin, and proteinaceous rodlets. This study is focused on the global importance of this outer layer. Single and multiple mutants without one to three major components of the outer layer were constructed and studied. The results showed that the absence of the target molecules resulting from multiple gene deletions led to unexpected phenotypes without any logical additivity. Unexpected compensatory cell wall surface modifications were indeed observed, such as the synthesis of the mycelial virulence factor galactosaminogalactan, the increase in chitin and glycoprotein concentration or particular changes in permeability. However, sensitivity of the multiple mutants to killing by phagocytic host cells confirmed the major importance of melanin in protecting conidia.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

ANR Project :

Amyloïdes fonctionnels formés par les hydrophobines du pathogène fongique Aspergillus fumigatus

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL