Legionella pneumophila Modulates Mitochondrial ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Title :
Legionella pneumophila Modulates Mitochondrial Dynamics to Trigger Metabolic Repurposing of Infected Macrophages
Author(s) :
Escoll, Pedro [Auteur]
Biologie des Bactéries intracellulaires - Biology of Intracellular Bacteria
Song, Ok-Ryul [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Viana, Flávia [Auteur]
Biologie des Bactéries intracellulaires - Biology of Intracellular Bacteria
Steiner, Bernhard [Auteur]
Institute of Medical Microbiology [Zurich]
Lagache, Thibault [Auteur]
Analyse d'images biologiques - Biological Image Analysis [BIA]
Olivo-Marin, Jean-Christophe [Auteur]
Analyse d'images biologiques - Biological Image Analysis [BIA]
Impens, Francis [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Brodin, Priscille [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Hilbi, Hubert [Auteur]
Institute of Medical Microbiology [Zurich]
Buchrieser, Carmen [Auteur correspondant]
Biologie des Bactéries intracellulaires - Biology of Intracellular Bacteria
Biologie des Bactéries intracellulaires - Biology of Intracellular Bacteria
Song, Ok-Ryul [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Viana, Flávia [Auteur]
Biologie des Bactéries intracellulaires - Biology of Intracellular Bacteria
Steiner, Bernhard [Auteur]
Institute of Medical Microbiology [Zurich]
Lagache, Thibault [Auteur]
Analyse d'images biologiques - Biological Image Analysis [BIA]
Olivo-Marin, Jean-Christophe [Auteur]
Analyse d'images biologiques - Biological Image Analysis [BIA]
Impens, Francis [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Brodin, Priscille [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Hilbi, Hubert [Auteur]
Institute of Medical Microbiology [Zurich]
Buchrieser, Carmen [Auteur correspondant]
Biologie des Bactéries intracellulaires - Biology of Intracellular Bacteria
Journal title :
Cell Host and Microbe
Pages :
302-316.e7
Publisher :
Elsevier
Publication date :
2017-09-13
ISSN :
1931-3128
English keyword(s) :
DNM1L
Legionella pneumophila
virulence
mitochondrial dynamics
live cell imaging
host-pathogen interactions
bioenergetics
Legionella pneumophila
virulence
mitochondrial dynamics
live cell imaging
host-pathogen interactions
bioenergetics
HAL domain(s) :
Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire
English abstract : [en]
The intracellular bacteria Legionella pneumophila encodes a type IV secretion system (T4SS) that injects effector proteins into macrophages in order to establish and replicate within the Legionella-containing vacuole (LCV). ...
Show more >The intracellular bacteria Legionella pneumophila encodes a type IV secretion system (T4SS) that injects effector proteins into macrophages in order to establish and replicate within the Legionella-containing vacuole (LCV). Once generated, the LCV interacts with mitochondria through unclear mechanisms. We show that Legionella uses both T4SS-independent and T4SS-dependent mechanisms to respectively interact with mitochondria and induce mitochondrial fragmentation that ultimately alters mitochondrial metabolism. The T4SS effector MitF, a Ran GTPase activator, is required for fission of the mitochondrial network. These effects of MitF occur through accumulation of mitochondrial DNM1L, a GTPase critical for fission. Furthermore mitochondrial respiration is abruptly halted in a T4SS-dependent manner, while T4SS-independent upregulation of cellular glycolysis remains elevated. Collectively, these alterations in mitochondrial dynamics promote a Warburg-like phenotype in macrophages that favors bacterial replication. Hence the rewiring of cellular bioenergetics to create a replication permissive niche in host cells is a virulence strategy of L. pneumophila.Show less >
Show more >The intracellular bacteria Legionella pneumophila encodes a type IV secretion system (T4SS) that injects effector proteins into macrophages in order to establish and replicate within the Legionella-containing vacuole (LCV). Once generated, the LCV interacts with mitochondria through unclear mechanisms. We show that Legionella uses both T4SS-independent and T4SS-dependent mechanisms to respectively interact with mitochondria and induce mitochondrial fragmentation that ultimately alters mitochondrial metabolism. The T4SS effector MitF, a Ran GTPase activator, is required for fission of the mitochondrial network. These effects of MitF occur through accumulation of mitochondrial DNM1L, a GTPase critical for fission. Furthermore mitochondrial respiration is abruptly halted in a T4SS-dependent manner, while T4SS-independent upregulation of cellular glycolysis remains elevated. Collectively, these alterations in mitochondrial dynamics promote a Warburg-like phenotype in macrophages that favors bacterial replication. Hence the rewiring of cellular bioenergetics to create a replication permissive niche in host cells is a virulence strategy of L. pneumophila.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
ANR Project :
Integrative Biology of Emerging Infectious Diseases
Eukaryotic genes in vacuolar pathogens and symbionts - Implications for virulence, metabolism and ecology
Développment d'une infrastructure française distribuée coordonnée
Plateau de microscopie de criblage à haut débit et d'analyse à très haute résolution
Eukaryotic genes in vacuolar pathogens and symbionts - Implications for virulence, metabolism and ecology
Développment d'une infrastructure française distribuée coordonnée
Plateau de microscopie de criblage à haut débit et d'analyse à très haute résolution
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