Title :

A combined computational fluid dynamics (CFD) and experimental approach to quantify the adhesion force of bacterial cells attached to a plane surface

Author(s) :

Boulbene, Benjamin [Auteur]
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés [LISBP]
Morchain, Jérôme [Auteur]
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés [LISBP]
Bonin, Muriel Mercier [Auteur]
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés [LISBP]
Janel, Sebastien [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Lafont, Frank [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Schmitz, Philippe [Auteur]
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés [LISBP]

Journal title :

AIChE Journal

Pages :

3614 - 3624

Publisher :

Wiley

Publication date :

2012

ISSN :

0001-1541

English keyword(s) :

bacterial adhesion
shear flow
hydrodynamics
TURBULENT PULSATING FLOWS
SHEAR-FLOW
STAINLESS-STEEL
SACCHAROMYCES-CEREVISIAE
BACILLUS SPORES
DETACHMENT KINETICS
MICROBIAL ADHESION
PLASMA DEPOSITION
INERT SURFACES
MODEL

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

A three-dimensional model is developed to study the laminar shear flow past a bacterial cell attached to a plane surface. The induced hydrodynamic forces and torque exerted on the cell are computed to clarify the prevailing ...
Show more >A three-dimensional model is developed to study the laminar shear flow past a bacterial cell attached to a plane surface. The induced hydrodynamic forces and torque exerted on the cell are computed to clarify the prevailing mechanisms involved in the detachment of model bacteria. Results are discussed in terms of drag and torque magnitude as a function of the angles defining the orientation of the cell. It is shown that reorientation and rolling of spheroid-shaped cells are favored. It is also confirmed that rod-shaped cells would tend to lie on the surface and become aligned with the flow. The model is used to quantify the adhesion force of spheroid Bacillus cereus spores to stainless steel, deduced from previously described experiments in a shear stress flow chamber. The magnitude of the predicted adhesion force is close to that obtained using atomic force microscopy under similar experimental conditions. (C) 2012 American Institute of Chemical Engineers AIChE J, 2012Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Non spécifiée

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL