Evaluation of Mass Spectrometry-Based ...
Type de document :
Article dans une revue scientifique
DOI :
PMID :
URL permanente :
Titre :
Evaluation of Mass Spectrometry-Based Detection of Panfungal Serum Disaccharide for Diagnosis of Invasive Fungal Infections: Results from a Collaborative Study Involving Six European Clinical Centers.
Auteur(s) :
Cornu, Marjorie [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Sendid, Boualem [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center (LIRIC) - U995
Mery, Alexandre [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Francois, Nadine [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Mikulska, Malgorzata [Auteur]
Letscher-Bru, Valérie [Auteur]
De Carolis, Elena [Auteur]
Damonti, Lauro [Auteur]
Titecat, Marie [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center (LIRIC) - U995
Bochud, Pierre-Yves [Auteur]
Alanio, Alexandre [Auteur]
Sanguinetti, Maurizio [Auteur]
Viscoli, Claudio [Auteur]
Herbrecht, Raoul [Auteur]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Poulain, Daniel [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center - U 995 [LIRIC]
Sendid, Boualem [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center (LIRIC) - U995
Mery, Alexandre [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Francois, Nadine [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Mikulska, Malgorzata [Auteur]
Letscher-Bru, Valérie [Auteur]
De Carolis, Elena [Auteur]
Damonti, Lauro [Auteur]
Titecat, Marie [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center (LIRIC) - U995
Bochud, Pierre-Yves [Auteur]
Alanio, Alexandre [Auteur]
Sanguinetti, Maurizio [Auteur]
Viscoli, Claudio [Auteur]
Herbrecht, Raoul [Auteur]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Poulain, Daniel [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Titre de la revue :
Journal of clinical microbiology
Nom court de la revue :
J Clin Microbiol
Numéro :
57
Date de publication :
2019-04-26
ISSN :
1098-660X
Mot(s)-clé(s) en anglais :
Adult
Aged
Aged, 80 and over
Antigens, Fungal
Aspergillosis
Candidiasis
Disaccharides
Europe
Female
Humans
Intersectoral Collaboration
Invasive Fungal Infections
Male
Mannans
Mass Spectrometry
Middle Aged
Mucormycosis
Sensitivity and Specificity
Young Adult
invasive aspergillosis
invasive candidiasis
invasive fungal infection
mass spectrometry
mucormycosis
serological diagnosis
Aged
Aged, 80 and over
Antigens, Fungal
Aspergillosis
Candidiasis
Disaccharides
Europe
Female
Humans
Intersectoral Collaboration
Invasive Fungal Infections
Male
Mannans
Mass Spectrometry
Middle Aged
Mucormycosis
Sensitivity and Specificity
Young Adult
invasive aspergillosis
invasive candidiasis
invasive fungal infection
mass spectrometry
mucormycosis
serological diagnosis
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
A mass spectrometry (MS) method that detects a serum disaccharide (DS) (MS-DS) was recently described for the diagnosis of invasive fungal infections (IFI). We carried out a European collaborative study to evaluate this ...
Lire la suite >A mass spectrometry (MS) method that detects a serum disaccharide (DS) (MS-DS) was recently described for the diagnosis of invasive fungal infections (IFI). We carried out a European collaborative study to evaluate this assay. Patients with the following IFI were selected according to the availability of sera obtained at about the time that IFI was documented: invasive candidiasis (IC; = 26 patients), invasive aspergillosis (IA; = 19), and mucormycosis (MM; = 23). Control sera originated from 20 neutropenic patients and 20 patients with bacteremia. MS-DS was carried out in blind manner for the diagnosis of IFI. A diagnosis of IC or IA was confirmed by detection of mannan (Man) or galactomannan (GM), respectively, associated with detection of (1,3)-β-d-glucan (BDG) in both infections. MM was detected by quantitative real-time PCR (qPCR). All tests discriminated sera from patients with IC from sera from control subjects with bacteremia ( ≤ 0.0009). For IC, the MS-DS sensitivity and specificity were 51% and 87%, respectively. MS-DS complemented the high specificity of Man monitoring. All tests discriminated sera from IA patients from sera from neutropenic controls ( ≤ 0.0009). For IA, MS-DS sensitivity and specificity were 64% and 95%, respectively. Only 13/36 serum samples from patients with MM were concordant by MS-DS and qPCR (6 were positive, and 7 were negative); 14 were positive by MS-DS alone. qPCR and MS-DS made a similar contribution to the diagnosis of MM. In patients undergoing long-term monitoring, the persistent circulation of serum disaccharide was observed, whereas DNA was detected only for a short period after initiation of treatment. MS-DS has an important role to play in the early diagnosis of IFI. Its panfungal nature and complementarity with other tests may justify its use in the management of IFI.Lire moins >
Lire la suite >A mass spectrometry (MS) method that detects a serum disaccharide (DS) (MS-DS) was recently described for the diagnosis of invasive fungal infections (IFI). We carried out a European collaborative study to evaluate this assay. Patients with the following IFI were selected according to the availability of sera obtained at about the time that IFI was documented: invasive candidiasis (IC; = 26 patients), invasive aspergillosis (IA; = 19), and mucormycosis (MM; = 23). Control sera originated from 20 neutropenic patients and 20 patients with bacteremia. MS-DS was carried out in blind manner for the diagnosis of IFI. A diagnosis of IC or IA was confirmed by detection of mannan (Man) or galactomannan (GM), respectively, associated with detection of (1,3)-β-d-glucan (BDG) in both infections. MM was detected by quantitative real-time PCR (qPCR). All tests discriminated sera from patients with IC from sera from control subjects with bacteremia ( ≤ 0.0009). For IC, the MS-DS sensitivity and specificity were 51% and 87%, respectively. MS-DS complemented the high specificity of Man monitoring. All tests discriminated sera from IA patients from sera from neutropenic controls ( ≤ 0.0009). For IA, MS-DS sensitivity and specificity were 64% and 95%, respectively. Only 13/36 serum samples from patients with MM were concordant by MS-DS and qPCR (6 were positive, and 7 were negative); 14 were positive by MS-DS alone. qPCR and MS-DS made a similar contribution to the diagnosis of MM. In patients undergoing long-term monitoring, the persistent circulation of serum disaccharide was observed, whereas DNA was detected only for a short period after initiation of treatment. MS-DS has an important role to play in the early diagnosis of IFI. Its panfungal nature and complementarity with other tests may justify its use in the management of IFI.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
Université de Lille
CNRS
CNRS
Équipe(s) de recherche :
Chemical Glycobiology
Date de dépôt :
2020-12-11T14:26:00Z
2020-12-15T14:37:06Z
2020-12-15T14:37:06Z
Fichiers
- P19.26 Journal of Clinical Microbiology-2019-Cornu-e01867-18.full.pdf
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