Titre :

Rev-erbalpha2 mRNA encodes a stable protein with a potential role in circadian clock regulation.

Auteur(s) :

Rambaud, Juliette [Auteur]
Laboratoire de Biologie Moléculaire de la Cellule [LBMC]
Triqueneaux, Gérard [Auteur]
Laboratoire de Biologie Moléculaire de la Cellule [LBMC]
Centre de génétique et de physiologie moléculaire et cellulaire [CGPhiMC]
Masse, Ingrid [Auteur]
Centre de génétique et de physiologie moléculaire et cellulaire [CGPhiMC]
Staels, Bart [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Laudet, Vincent [Auteur]
Institut de Génomique Fonctionnelle de Lyon [IGFL]
Benoit, Gérard [Auteur]
Centre de génétique et de physiologie moléculaire et cellulaire [CGPhiMC]

Titre de la revue :

Mol Endocrinol

Pagination :

630-9

Date de publication :

2009-05

Mot(s)-clé(s) :

Male
Immunoprecipitation
COS Cells
Messenger
Receptors
Transcription

Mot(s)-clé(s) en anglais :

Animals
Blotting
Western
Cercopithecus aethiops
Circadian Rhythm
Computational Biology
DNA-Binding Proteins
Electrophoretic Mobility Shift Assay
Gene Expression Regulation
Mice
Nuclear Receptor Subfamily 1
Group D
Member 1
Promoter Regions
Genetic
Protein Isoforms
Protein Multimerization
RNA
Cytoplasmic and Nuclear
Reverse Transcriptase Polymerase Chain Reaction

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire

Résumé en anglais : [en]

Circadian rhythms are observed in nearly all aspects of physiology and behavior. In mammals, such biological rhythms are supported by a complex network of self-sustained transcriptional loops and posttranslational ...
Lire la suite >Circadian rhythms are observed in nearly all aspects of physiology and behavior. In mammals, such biological rhythms are supported by a complex network of self-sustained transcriptional loops and posttranslational modifications, which regulate timely controlled production and degradation of critical factors on a 24-h basis. Among these factors, the orphan nuclear receptor rev-erbalpha plays an essential role by linking together positive and negative regulatory loops. As an essential part of the circadian core clock mechanism, REV-ERBalpha expression shows a precisely scheduled oscillation reflecting the tight control of its production and degradation. In previous studies, we identified two alternative transcripts encoding two protein variants referred to as REV-ERBalpha1 and -alpha2. Interestingly, recent work identified structural elements present only in REV-ERBalpha1 that controls its turnover and thereby influences circadian oscillations. In the present work, we comparatively analyze the two variants and show that REV-ERBalpha2 exhibits a half-life incompatible with a circadian function, suggesting that this variant exerts different biological functions. However, our comparative study clearly indicates undistinguishable DNA-binding properties and transcriptional repression activity as well as a similar regulation mechanism. The only consistent difference appears to be the relative expression level of the two transcripts, rev-erbalpha1 being one to 100 times more expressed than alpha2 depending on tissue and circadian time. Taking this finding into consideration, we reassessed REV-ERBalpha2 turnover and were able to show that this variant exhibits a reduced half-life when coexpressed with REV-ERBalpha1. We propose that the relative expression levels of the two REV-ERBalpha variants fine-tune the circadian period length by regulating REV-ERBalpha half-life.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Récepteurs nucléaires, Maladies Cardiovasculaires et Diabète (RNMCD) - U1011

Source :

Harvested from HAL