Docosahexaenoic fatty acid-containing ...
Document type :
Pré-publication ou Document de travail
Title :
Docosahexaenoic fatty acid-containing phospholipids affect plasma membrane susceptibility to disruption by bacterial toxin-induced macroapertures
Author(s) :
Tsai, Meng-Chen [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Toxines bactériennes - Bacterial Toxins
Fleuriot, Lucile [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Janel, Sébastien [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Gonzalez-Rodriguez, David [Auteur]
Laboratoire de Chimie et Physique - Approche Multi-échelle des Milieux Complexes [LCP-A2MC]
Morel, Camille [Auteur]
Toxines bactériennes - Bacterial Toxins
Université Paris Diderot, Sorbonne Paris Cité, Paris, France
Mettouchi, Amel [Auteur]
Toxines bactériennes - Bacterial Toxins
Université Paris Diderot, Sorbonne Paris Cité, Paris, France
Debayle, Delphine [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Dallongeville, Stéphane [Auteur]
Analyse d'images biologiques - Biological Image Analysis [BIA]
Olivo-Marin, Jean-Christophe [Auteur]
Analyse d'images biologiques - Biological Image Analysis [BIA]
Antonny, Bruno [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Lafont, Frank [Auteur]
Laboratoire de Chimie et Physique - Approche Multi-échelle des Milieux Complexes [LCP-A2MC]
Lemichez, Emmanuel [Auteur correspondant]
Toxines bactériennes - Bacterial Toxins
Barelli, Hélène [Auteur correspondant]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Toxines bactériennes - Bacterial Toxins
Fleuriot, Lucile [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Janel, Sébastien [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Gonzalez-Rodriguez, David [Auteur]
Laboratoire de Chimie et Physique - Approche Multi-échelle des Milieux Complexes [LCP-A2MC]
Morel, Camille [Auteur]
Toxines bactériennes - Bacterial Toxins
Université Paris Diderot, Sorbonne Paris Cité, Paris, France
Mettouchi, Amel [Auteur]
Toxines bactériennes - Bacterial Toxins
Université Paris Diderot, Sorbonne Paris Cité, Paris, France
Debayle, Delphine [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Dallongeville, Stéphane [Auteur]
Analyse d'images biologiques - Biological Image Analysis [BIA]
Olivo-Marin, Jean-Christophe [Auteur]
Analyse d'images biologiques - Biological Image Analysis [BIA]
Antonny, Bruno [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Lafont, Frank [Auteur]
Laboratoire de Chimie et Physique - Approche Multi-échelle des Milieux Complexes [LCP-A2MC]
Lemichez, Emmanuel [Auteur correspondant]
Toxines bactériennes - Bacterial Toxins
Barelli, Hélène [Auteur correspondant]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
HAL domain(s) :
Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie
Sciences du Vivant [q-bio]/Biologie cellulaire
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie
Sciences du Vivant [q-bio]/Biologie cellulaire
English abstract : [en]
Metabolic studies and animal knockout models point to the critical role of polyunsaturated docosahexaenoic acid (22:6, DHA)-containing phospholipids (PLs) in physiology. Here, we study the impact of DHA-PLs on the dynamics ...
Show more >Metabolic studies and animal knockout models point to the critical role of polyunsaturated docosahexaenoic acid (22:6, DHA)-containing phospholipids (PLs) in physiology. Here, we study the impact of DHA-PLs on the dynamics of transendothelial cell macroapertures (TEMs) tunnels triggered by the RhoA GTPase inhibitory exotoxin C3 from Clostridium botulinum . Through lipidomic analyses, we show that primary human umbilical vein endothelial cells (HUVECs) subjected to DHA-diet undergo a 6-fold DHA-PLs enrichment in plasma membrane at the expense of monounsaturated OA-PLs. In contrast, OA-diet had almost no effect on PLs composition. Consequently, DHA treatment increases the nucleation rate of TEMs by 2-fold that we ascribe to a reduction of cell thickness. We reveal that the global transcellular area of cells remains conserved through a reduction of the width and lifetime of TEMs. Altogether, we reveal a homeostasis between plasma membrane DHA-PLs content and large-scale membrane dynamics.Show less >
Show more >Metabolic studies and animal knockout models point to the critical role of polyunsaturated docosahexaenoic acid (22:6, DHA)-containing phospholipids (PLs) in physiology. Here, we study the impact of DHA-PLs on the dynamics of transendothelial cell macroapertures (TEMs) tunnels triggered by the RhoA GTPase inhibitory exotoxin C3 from Clostridium botulinum . Through lipidomic analyses, we show that primary human umbilical vein endothelial cells (HUVECs) subjected to DHA-diet undergo a 6-fold DHA-PLs enrichment in plasma membrane at the expense of monounsaturated OA-PLs. In contrast, OA-diet had almost no effect on PLs composition. Consequently, DHA treatment increases the nucleation rate of TEMs by 2-fold that we ascribe to a reduction of cell thickness. We reveal that the global transcellular area of cells remains conserved through a reduction of the width and lifetime of TEMs. Altogether, we reveal a homeostasis between plasma membrane DHA-PLs content and large-scale membrane dynamics.Show less >
Language :
Anglais
ANR Project :
Source :
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