Nutritionally induced tanycytic plasticity in the hypothalamus of adult ewes

Prezotto, L. D.; Thorson, J. F.; Prevot, V.; Redmer, D. A.; Grazul-Bilska, A. T.

Document type :

Article dans une revue scientifique: Article original

DOI :

10.1016/j.domaniend.2020.106438

PMID :

32388344

Permalink :

http://hdl.handle.net/20.500.12210/40242

Title :

Nutritionally induced tanycytic plasticity in the hypothalamus of adult ewes

Author(s) :

Prezotto, L. D. [Auteur]
Thorson, J. F. [Auteur]
Prevot, V. [Auteur]
Redmer, D. A. [Auteur]
Grazul-Bilska, A. T. [Auteur]

Journal title :

Domestic animal endocrinology

Abbreviated title :

Domest. Anim. Endocrinol.

Volume number :

Pages :

106438

Publication date :

2020-03-26

ISSN :

1879-0054

Keyword(s) :

Nutrition
Hypothalamus
Ewe
Blood-brain barrier
Tanycyte
Proopiomelanocortin

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The blood-brain barrier regulates the transport of molecules that convey global energetic status to the feeding circuitry within the hypothalamus. Capillaries within the median eminence (ME) and tight junctions between ...
Show more >The blood-brain barrier regulates the transport of molecules that convey global energetic status to the feeding circuitry within the hypothalamus. Capillaries within the median eminence (ME) and tight junctions between tanycytes lining the third ventricle (3V) are critical components of this barrier. Herein, we tested the hypothesis that altering the plane of nutrition results in the structural reorganization of tanycytes, tight junctions, and capillary structure within the medial basal hypothalamus. Proopiomelanocortin (POMC) neuronal content within the arcuate nucleus of the hypothalamus (ARC) was also assessed to test whether reduced nutritional status improved access of nutrients to the ARC, while decreasing the access of nutrients of overfed animals. Multiparous, nongestating ewes were stratified by weight and randomly assigned to dietary treatments offered for 75 d: 200% of dietary recommendations (overfed), 100% of dietary recommendations (control), or 60% of dietary recommendations (underfed). The number of POMC-expressing neurons within the ARC was increased (P ≤ 0.002) in underfed ewes. Overfeeding increased (P ≤ 0.01) tanycyte cellular process penetration and density compared with control and underfeeding as assessed using vimentin immunostaining. Immunostaining of tight junctions along the wall of the 3V did not differ (P = 0.32) between treatments. No differences were observed in capillary density (P = 0.21) or classification (P ≥ 0.47) within the ME. These results implicate that changes within the satiety center and morphology of tanycytes within the ARC occur as an adaptation to nutrient availability.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Inserm
Université de Lille

Collections :

Lille Neurosciences & Cognition (LilNCog) - U 1172

Research team(s) :

Développement et plasticité du cerveau neuro-endocrine

Submission date :

2021-06-23T13:46:12Z

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