Hydroxychloroquine levels in patients with ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Permalink :
Title :
Hydroxychloroquine levels in patients with systemic lupus erythematosus: whole blood is preferable but serum levels also detect non-adherence
Author(s) :
Blanchet, Benoit [Auteur]
Jallouli, Moez [Auteur]
Allard, Marie [Auteur]
Ghillani-Dalbin, Pascale [Auteur]
Galicier, Lionel [Auteur]
Aumaitre, Olivier [Auteur]
Chasset, Francois [Auteur]
Le Guern, Véronique [Auteur]
Liote, Frederic [Auteur]
Smail, Amar [Auteur]
Limal, Nicolas [Auteur]
Perard, Laurent [Auteur]
Desmurs-Clavel, Helene [Auteur]
Le Thi Huong, Du [Auteur]
Asli, Bouchra [Auteur]
Kahn, Jean-Emmannuel [Auteur]
Sailler, Laurent [Auteur]
Ackermann, Felix [Auteur]
Papo, Thomas [Auteur]
Sacre, Karim [Auteur]
Fain, Olivier [Auteur]
Stirnemann, Jerome [Auteur]
Cacoub, Patrice [Auteur]
Leroux, Gaelle [Auteur]
Cohen-Bittan, Judith [Auteur]
Sellam, Jeremie [Auteur]
Mariette, Xavier [Auteur]
Goulvestre, Claire [Auteur]
Hulot, Jean Sebastien [Auteur]
Amoura, Zahir [Auteur]
Vidal, Michel [Auteur]
Piette, Jean-Charles [Auteur]
Jourde-Chiche, Noémie [Auteur]
Costedoat-Chalumeau, Nathalie [Auteur]
Jallouli, Moez [Auteur]
Allard, Marie [Auteur]
Ghillani-Dalbin, Pascale [Auteur]
Galicier, Lionel [Auteur]
Aumaitre, Olivier [Auteur]
Chasset, Francois [Auteur]
Le Guern, Véronique [Auteur]
Liote, Frederic [Auteur]
Smail, Amar [Auteur]
Limal, Nicolas [Auteur]
Perard, Laurent [Auteur]
Desmurs-Clavel, Helene [Auteur]
Le Thi Huong, Du [Auteur]
Asli, Bouchra [Auteur]
Kahn, Jean-Emmannuel [Auteur]

Sailler, Laurent [Auteur]
Ackermann, Felix [Auteur]
Papo, Thomas [Auteur]
Sacre, Karim [Auteur]
Fain, Olivier [Auteur]
Stirnemann, Jerome [Auteur]
Cacoub, Patrice [Auteur]
Leroux, Gaelle [Auteur]
Cohen-Bittan, Judith [Auteur]
Sellam, Jeremie [Auteur]
Mariette, Xavier [Auteur]
Goulvestre, Claire [Auteur]
Hulot, Jean Sebastien [Auteur]
Amoura, Zahir [Auteur]
Vidal, Michel [Auteur]
Piette, Jean-Charles [Auteur]
Jourde-Chiche, Noémie [Auteur]
Costedoat-Chalumeau, Nathalie [Auteur]
Journal title :
Arthritis research & therapy
Abbreviated title :
null
Volume number :
22
Pages :
223
Publication date :
2020-09-25
ISSN :
1478-6362
Keyword(s) :
Systemic lupus erythematosus
Hydroxychloroquine
Adherence
Drug monitoring
Serum
Hydroxychloroquine
Adherence
Drug monitoring
Serum
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Hydroxychloroquine (HCQ) levels can be measured in both serum and whole blood. No cut-off point for non-adherence has been established in serum nor have these methods ever been compared. The aims of this study were to ...
Show more >Hydroxychloroquine (HCQ) levels can be measured in both serum and whole blood. No cut-off point for non-adherence has been established in serum nor have these methods ever been compared. The aims of this study were to compare these two approaches and determine if serum HCQ cut-off points can be established to identify non-adherent patients. HCQ levels were measured in serum and whole blood from 573 patients with systemic lupus erythematosus (SLE). The risk factors for active SLE (SLEDAI score > 4) were identified by multiple logistic regression. Serum HCQ levels were measured in 68 additional patients known to be non-adherent, i.e. with whole-blood HCQ < 200 ng/mL. The mean (± SD) HCQ levels were 469 ± 223 ng/mL in serum and 916 ± 449 ng/mL in whole blood. The mean ratio of serum/whole-blood HCQ levels was 0.53 ± 0.15. In the multivariate analysis, low whole-blood HCQ levels (P = 0.023), but not serum HCQ levels, were independently associated with active SLE. From the mean serum/whole-blood level ratio, a serum HCQ level of 106 ng/mL was extrapolated as the corresponding cut-off to identify non-adherent patients with a sensitivity of 0.87 (95% CI 0.76-0.94) and specificity of 0.89 (95% CI 0.72-0.98). All serum HCQ levels of patients with whole-blood HCQ below the detectable level (< 20 ng/mL) were also undetectable (< 20 ng/mL). These data suggest that whole blood is better than serum for assessing the pharmacokinetic/pharmacodynamic relation of HCQ. Our results support the use of serum HCQ levels to assess non-adherence when whole blood is unavailable.Show less >
Show more >Hydroxychloroquine (HCQ) levels can be measured in both serum and whole blood. No cut-off point for non-adherence has been established in serum nor have these methods ever been compared. The aims of this study were to compare these two approaches and determine if serum HCQ cut-off points can be established to identify non-adherent patients. HCQ levels were measured in serum and whole blood from 573 patients with systemic lupus erythematosus (SLE). The risk factors for active SLE (SLEDAI score > 4) were identified by multiple logistic regression. Serum HCQ levels were measured in 68 additional patients known to be non-adherent, i.e. with whole-blood HCQ < 200 ng/mL. The mean (± SD) HCQ levels were 469 ± 223 ng/mL in serum and 916 ± 449 ng/mL in whole blood. The mean ratio of serum/whole-blood HCQ levels was 0.53 ± 0.15. In the multivariate analysis, low whole-blood HCQ levels (P = 0.023), but not serum HCQ levels, were independently associated with active SLE. From the mean serum/whole-blood level ratio, a serum HCQ level of 106 ng/mL was extrapolated as the corresponding cut-off to identify non-adherent patients with a sensitivity of 0.87 (95% CI 0.76-0.94) and specificity of 0.89 (95% CI 0.72-0.98). All serum HCQ levels of patients with whole-blood HCQ below the detectable level (< 20 ng/mL) were also undetectable (< 20 ng/mL). These data suggest that whole blood is better than serum for assessing the pharmacokinetic/pharmacodynamic relation of HCQ. Our results support the use of serum HCQ levels to assess non-adherence when whole blood is unavailable.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
CHU Lille
Inserm
Université de Lille
Inserm
Université de Lille
Submission date :
2021-07-06T12:48:08Z