Monoliths for microfluidic devices in proteomics
Type de document :
Compte-rendu et recension critique d'ouvrage
Titre :
Monoliths for microfluidic devices in proteomics
Auteur(s) :
Le Gac, S. [Auteur]
Carlier, Julien [Auteur]
Matériaux et Acoustiques pour MIcro et NAno systèmes intégrés - IEMN [MAMINA - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Camart, Jean-Christophe [Auteur]
Cren-Olivé, C. [Auteur]
Rolando, C. [Auteur]
Carlier, Julien [Auteur]
Matériaux et Acoustiques pour MIcro et NAno systèmes intégrés - IEMN [MAMINA - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Camart, Jean-Christophe [Auteur]
Cren-Olivé, C. [Auteur]
Rolando, C. [Auteur]
Titre de la revue :
Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences
Pagination :
3-14
Éditeur :
Elsevier
Date de publication :
2004
ISSN :
1570-0232
Mot(s)-clé(s) en anglais :
AcCN
acetonitrile
AIBN
cyclohexanol
CyOH
Cyt C
Cytochrome C
EDMA
EG
ethylene dimethacrylate
ethylene glycol
formic acid
HCOOH
high voltage
HV
IDA
information dependant acquisition
lauryl methacrylate
LMA
MeOH
acetonitrile
AIBN
cyclohexanol
CyOH
Cyt C
Cytochrome C
EDMA
EG
ethylene dimethacrylate
ethylene glycol
formic acid
HCOOH
high voltage
HV
IDA
information dependant acquisition
lauryl methacrylate
LMA
MeOH
Discipline(s) HAL :
Sciences de l'ingénieur [physics]
Résumé en anglais : [en]
We report here on the preparation of monolithic capillary columns in view to their integration in a microsystem for on-chip sample preparation before their on-line analysis by electrospray and mass spectrometry (ESI-MS). ...
Lire la suite >We report here on the preparation of monolithic capillary columns in view to their integration in a microsystem for on-chip sample preparation before their on-line analysis by electrospray and mass spectrometry (ESI-MS). These monolithic columns are based on polymer materials and consist of reverse phases for peptide separation and/or desalting. They were prepared using lauryl methacrylate (LMA), ethylene dimethacrylate (EDMA) as well as a suitable porogenic mixture composed of cyclohexanol and ethylene glycol. The resulting stationary phases present thus a C12-functionality. The LMA-based columns were first prepared in a capillary format using capillary tubing of 75 μm i.d. and tested in nanoLC-MS experiments for the separation of a commercial Cytochrome C digest composed of 12 peptidic fragments whose isoelectric point values and hydrophobic character cover a wide range. The LMA-based columns were capable of separating the peptidic fragments and their performances were seen to be similar as those of standard commercial columns dedicated to proteomic purposes with calculated separation efficiencies up to 145×103 plates/m. Monolithic LMA-based phases were then successfully polymerized in microchannels fabricated using the negative photoresist SU-8. After the polymerization, the systems were seen to withstand the pressures applied during the nanoLC-MS separation tests that were carried out in the same conditions as for the monolithic capillary columns. The pressure drop during these tests of the in-microchannel monoliths was as high as 50 bar; however, the separation was not as good as for a capillary format which could be accounted for by the monolith dimensions.Lire moins >
Lire la suite >We report here on the preparation of monolithic capillary columns in view to their integration in a microsystem for on-chip sample preparation before their on-line analysis by electrospray and mass spectrometry (ESI-MS). These monolithic columns are based on polymer materials and consist of reverse phases for peptide separation and/or desalting. They were prepared using lauryl methacrylate (LMA), ethylene dimethacrylate (EDMA) as well as a suitable porogenic mixture composed of cyclohexanol and ethylene glycol. The resulting stationary phases present thus a C12-functionality. The LMA-based columns were first prepared in a capillary format using capillary tubing of 75 μm i.d. and tested in nanoLC-MS experiments for the separation of a commercial Cytochrome C digest composed of 12 peptidic fragments whose isoelectric point values and hydrophobic character cover a wide range. The LMA-based columns were capable of separating the peptidic fragments and their performances were seen to be similar as those of standard commercial columns dedicated to proteomic purposes with calculated separation efficiencies up to 145×103 plates/m. Monolithic LMA-based phases were then successfully polymerized in microchannels fabricated using the negative photoresist SU-8. After the polymerization, the systems were seen to withstand the pressures applied during the nanoLC-MS separation tests that were carried out in the same conditions as for the monolithic capillary columns. The pressure drop during these tests of the in-microchannel monoliths was as high as 50 bar; however, the separation was not as good as for a capillary format which could be accounted for by the monolith dimensions.Lire moins >
Langue :
Anglais
Vulgarisation :
Non
Source :
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