Title :

L. plantarum WCFS1 enhances Treg frequencies by activating DCs even in absence of sampling of bacteria in the Peyer Patches

Author(s) :

Bermudez-Brito, Miriam [Auteur correspondant]
University of Groningen [Groningen]
University Medical Center Groningen [Groningen] [UMCG]
Top Institute Food and Nutrition [TIFN]
Borghuis, Theo [Auteur]
University of Groningen [Groningen]
University Medical Center Groningen [Groningen] [UMCG]
Daniel, Catherine [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Pot, Bruno [Auteur]
de Haan, Bart [Auteur]
University of Groningen [Groningen]
University Medical Center Groningen [Groningen] [UMCG]
Faas, Marijke [Auteur]
University of Groningen [Groningen]
University Medical Center Groningen [Groningen] [UMCG]
de Vos, Paul [Auteur]
University of Groningen [Groningen]
University Medical Center Groningen [Groningen] [UMCG]
Top Institute Food and Nutrition [TIFN]

Journal title :

SCIENTIFIC REPORTS

Pages :

1785

Publisher :

Nature Publishing Group

Publication date :

2018

ISSN :

2045-2322

HAL domain(s) :

Sciences du Vivant [q-bio]/Alimentation et Nutrition
Sciences du Vivant [q-bio]/Immunologie/Immunothérapie

English abstract : [en]

Probiotics such as L. plantarum WCFS1 can modulate immune responses in healthy subjects but how this occurs is still largely unknown. Immune-sampling in the Peyer Patches has been suggested to be one of the mechanisms. ...
Show more >Probiotics such as L. plantarum WCFS1 can modulate immune responses in healthy subjects but how this occurs is still largely unknown. Immune-sampling in the Peyer Patches has been suggested to be one of the mechanisms. Here we studied the systemic and intestinal immune effects in combination with a trafficking study through the intestine of a well-established immunomodulating probiotic, i.e. L. plantarum WCFS1. We demonstrate that not more than 2–3 bacteria were sampled and in many animals not any bacterium could be found in the PP. Despite this, L. plantarum was associated with a strong increase in infiltration of regulatory CD103+ DCs and generation of regulatory T cells in the spleen. Also, a reduced splenic T helper cell cytokine response was observed after ex vivo restimulation. L. plantarum enhanced Treg cells and attenuated the T helper 2 response in healthy mice. We demonstrate that, in healthy mice, immune sampling is a rare phenomenon and not required for immunomodulation. Also in absence of any sampling immune activation was found illustrating that host-microbe interaction on the Peyer Patches was enough to induce immunomodulation of DCs and T-cells.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL