Proanthocyanidin microcapsules: preparation, ...
Document type :
Article dans une revue scientifique
Permalink :
Title :
Proanthocyanidin microcapsules: preparation, properties and free radical scavenging activity
Author(s) :
Andry, M.-C [Auteur]
Université de Reims Champagne-Ardenne [URCA]
Vezin, Herve [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Dumistracel, I [Auteur]
Université de Reims Champagne-Ardenne [URCA]
Bernier, Jean-Luc [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Lévy, M.-C [Auteur]
Université de Reims Champagne-Ardenne [URCA]
Université de Reims Champagne-Ardenne [URCA]
Vezin, Herve [Auteur]

Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Dumistracel, I [Auteur]
Université de Reims Champagne-Ardenne [URCA]
Bernier, Jean-Luc [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Lévy, M.-C [Auteur]
Université de Reims Champagne-Ardenne [URCA]
Journal title :
International Journal of Pharmaceutics
Volume number :
171
Pages :
217-226
Publisher :
Elsevier BV
Publication date :
1998-09-15
ISSN :
0378-5173
English keyword(s) :
Microcapsule
Proanthocyanidin
Interfacial cross-linking
Terephthaloyl chloride
Free radical scavenging activity
EPR
Proanthocyanidin
Interfacial cross-linking
Terephthaloyl chloride
Free radical scavenging activity
EPR
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Microcapsules were prepared by interfacial cross-linking of grape proanthocyanidin (GPO) with terephthaloyl chloride (TC). Using 5% TC, 30-min reaction time and a stirring speed of 5000 rpm, batches were prepared from 10% ...
Show more >Microcapsules were prepared by interfacial cross-linking of grape proanthocyanidin (GPO) with terephthaloyl chloride (TC). Using 5% TC, 30-min reaction time and a stirring speed of 5000 rpm, batches were prepared from 10% GPO solutions in buffers pH 8, 9.8, and 11, respectively. Microcapsules were studied with respect to morphology (microscopy), size (laser diffraction technique), stability in water at 45°C and, for stable batches, IR spectroscopy, degradation in human plasma, and free radical scavenging activity studied by electron paramagnetic resonance (EPR) using both extinction of the stable radical DPPH (2,2-diphenyl-picryl-hydrazyl), and scavenging of OH° (evaluated using 5,5-dimethyl-pyrrolidine-N-oxide; DMPO). All particles had a mean size <10 μm. Microcapsules obtained at pH 8 were unstable (yellow supernatant after 8 days). Microcapsules obtained at pH 9.8 and 11 exhibited a high stability (>5 months). Using a stirring speed of 3000 rpm at pH 11 provided 15-μm particles which gave a yellow supernatant after 3 weeks. The IR spectra of the two stable batches (pH 9.8 and 11) showed bands at 1736 and 1267 cm−1 reflecting the formation of esters. They were slowly degraded in plasma, and although less active than the initial GPO, they exhibited a significant antioxidant activity. The method appears to be an efficient means of stabilizing the polyphenol without suppressing the free radical scavenging activity.Show less >
Show more >Microcapsules were prepared by interfacial cross-linking of grape proanthocyanidin (GPO) with terephthaloyl chloride (TC). Using 5% TC, 30-min reaction time and a stirring speed of 5000 rpm, batches were prepared from 10% GPO solutions in buffers pH 8, 9.8, and 11, respectively. Microcapsules were studied with respect to morphology (microscopy), size (laser diffraction technique), stability in water at 45°C and, for stable batches, IR spectroscopy, degradation in human plasma, and free radical scavenging activity studied by electron paramagnetic resonance (EPR) using both extinction of the stable radical DPPH (2,2-diphenyl-picryl-hydrazyl), and scavenging of OH° (evaluated using 5,5-dimethyl-pyrrolidine-N-oxide; DMPO). All particles had a mean size <10 μm. Microcapsules obtained at pH 8 were unstable (yellow supernatant after 8 days). Microcapsules obtained at pH 9.8 and 11 exhibited a high stability (>5 months). Using a stirring speed of 3000 rpm at pH 11 provided 15-μm particles which gave a yellow supernatant after 3 weeks. The IR spectra of the two stable batches (pH 9.8 and 11) showed bands at 1736 and 1267 cm−1 reflecting the formation of esters. They were slowly degraded in plasma, and although less active than the initial GPO, they exhibited a significant antioxidant activity. The method appears to be an efficient means of stabilizing the polyphenol without suppressing the free radical scavenging activity.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Collections :
Submission date :
2021-11-03T11:13:34Z
2021-11-10T08:41:42Z
2021-11-10T08:41:42Z
Files
- Andry et al. - 1998 - Proanthocyanidin microcapsules Preparation, properties and free radical scavenging activity.pdf
- Version éditeur
- Confidential access
- Access the document