Title :

Proanthocyanidin microcapsules: preparation, properties and free radical scavenging activity

Author(s) :

Andry, M.-C [Auteur]
Université de Reims Champagne-Ardenne [URCA]
Vezin, Herve [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Dumistracel, I [Auteur]
Université de Reims Champagne-Ardenne [URCA]
Bernier, Jean-Luc [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Lévy, M.-C [Auteur]
Université de Reims Champagne-Ardenne [URCA]

Journal title :

International Journal of Pharmaceutics

Volume number :

171

Pages :

217-226

Publisher :

Elsevier BV

Publication date :

1998-09-15

ISSN :

0378-5173

English keyword(s) :

Microcapsule
Proanthocyanidin
Interfacial cross-linking
Terephthaloyl chloride
Free radical scavenging activity
EPR

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Microcapsules were prepared by interfacial cross-linking of grape proanthocyanidin (GPO) with terephthaloyl chloride (TC). Using 5% TC, 30-min reaction time and a stirring speed of 5000 rpm, batches were prepared from 10% ...
Show more >Microcapsules were prepared by interfacial cross-linking of grape proanthocyanidin (GPO) with terephthaloyl chloride (TC). Using 5% TC, 30-min reaction time and a stirring speed of 5000 rpm, batches were prepared from 10% GPO solutions in buffers pH 8, 9.8, and 11, respectively. Microcapsules were studied with respect to morphology (microscopy), size (laser diffraction technique), stability in water at 45°C and, for stable batches, IR spectroscopy, degradation in human plasma, and free radical scavenging activity studied by electron paramagnetic resonance (EPR) using both extinction of the stable radical DPPH (2,2-diphenyl-picryl-hydrazyl), and scavenging of OH° (evaluated using 5,5-dimethyl-pyrrolidine-N-oxide; DMPO). All particles had a mean size <10 μm. Microcapsules obtained at pH 8 were unstable (yellow supernatant after 8 days). Microcapsules obtained at pH 9.8 and 11 exhibited a high stability (>5 months). Using a stirring speed of 3000 rpm at pH 11 provided 15-μm particles which gave a yellow supernatant after 3 weeks. The IR spectra of the two stable batches (pH 9.8 and 11) showed bands at 1736 and 1267 cm−1 reflecting the formation of esters. They were slowly degraded in plasma, and although less active than the initial GPO, they exhibited a significant antioxidant activity. The method appears to be an efficient means of stabilizing the polyphenol without suppressing the free radical scavenging activity.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Autres travaux scientifiques

Submission date :

2021-11-03T11:13:34Z
2021-11-10T08:41:42Z