DNA cleavage by hydroxy-salicylidene-eth ...
Type de document :
Article dans une revue scientifique
DOI :
URL permanente :
Titre :
DNA cleavage by hydroxy-salicylidene-ethylendiamine-iron complexes
Auteur(s) :
Routier, Sylvain [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Vezin, Herve [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Lamour, Eric [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Bernier, Jean-Luc [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Catteau, Jean-Pierre [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Bailly, Christian [Auteur]
Université de Lille
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Vezin, Herve [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Lamour, Eric [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Bernier, Jean-Luc [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Catteau, Jean-Pierre [Auteur]
Ecole Nationale Supérieure de Chimie de Lille [ENSCL]
Bailly, Christian [Auteur]
Université de Lille
Titre de la revue :
Nucleic acids research
Numéro :
27
Pagination :
4160-4166
Éditeur :
Oxford University Press (OUP)
Date de publication :
1999-11-01
ISSN :
1362-4962
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
Bis(hydroxy)salen Fe complexes were designed as self-activated chemical nucleases. The presence of a hy-droxyl group on the two salicylidene moieties serve to form a hydroquinone system cooperating with the iron redox ...
Lire la suite >Bis(hydroxy)salen Fe complexes were designed as self-activated chemical nucleases. The presence of a hy-droxyl group on the two salicylidene moieties serve to form a hydroquinone system cooperating with the iron redox system to facilitate spontaneous formation of free radicals. We compared the DNA binding and cleaving properties of the ortho-, mete- and para-(bishydroxy) salen Fe complexes with that of the corresponding chelate lacking the hydroxyl groups. DNA melting temperature studies indicated that the para complex exhibits the highest affinity for DNA. In addition, this para compound was considerably more potent at cleaving supercoiled plasmid DNA than the regio-isomeric ortho- and meta-hydroxy-salen·Fe complexes, even in the absence of a reducing agent, such as dithiothreitol used to activate the metal complex. The DNA cleaving activity of the para isomer is both time and concentration dependent and the complexed iron atom is absolutely essential for the sequence uniform cleavage of DNA. From a mechanistic point of view, electron spin resonance measurements suggest that DNA contributes positively to the activation of the semi-quinone system and the production of ligand radical species responsible for subsequent strand scission in the absence of a reducing agent. The para-hydroxy-salen-Fe complex has been used for detecting sequence-specific drug-DNA interactions. Specific binding of Hoechst 33258 to AT sequences and chromomycin to GC sequences were shown. The para -bis(hydroxy)salenFe derivative complements the tool box of footprinting reagents which can be utilised to produce efficient cleavage of DNA.Lire moins >
Lire la suite >Bis(hydroxy)salen Fe complexes were designed as self-activated chemical nucleases. The presence of a hy-droxyl group on the two salicylidene moieties serve to form a hydroquinone system cooperating with the iron redox system to facilitate spontaneous formation of free radicals. We compared the DNA binding and cleaving properties of the ortho-, mete- and para-(bishydroxy) salen Fe complexes with that of the corresponding chelate lacking the hydroxyl groups. DNA melting temperature studies indicated that the para complex exhibits the highest affinity for DNA. In addition, this para compound was considerably more potent at cleaving supercoiled plasmid DNA than the regio-isomeric ortho- and meta-hydroxy-salen·Fe complexes, even in the absence of a reducing agent, such as dithiothreitol used to activate the metal complex. The DNA cleaving activity of the para isomer is both time and concentration dependent and the complexed iron atom is absolutely essential for the sequence uniform cleavage of DNA. From a mechanistic point of view, electron spin resonance measurements suggest that DNA contributes positively to the activation of the semi-quinone system and the production of ligand radical species responsible for subsequent strand scission in the absence of a reducing agent. The para-hydroxy-salen-Fe complex has been used for detecting sequence-specific drug-DNA interactions. Specific binding of Hoechst 33258 to AT sequences and chromomycin to GC sequences were shown. The para -bis(hydroxy)salenFe derivative complements the tool box of footprinting reagents which can be utilised to produce efficient cleavage of DNA.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Vulgarisation :
Non
Collections :
Date de dépôt :
2021-11-03T11:18:41Z
2021-11-10T08:47:14Z
2021-11-10T08:47:14Z