Title :

Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography

Author(s) :

Coquelle, Nicolas [Auteur]
Université Grenoble Alpes [UGA]
Sliwa, Michel [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Woodhouse, Joyce [Auteur]
Institut de biologie structurale [IBS - UMR 5075]
Schirò, Giorgio [Auteur]
Institut de biologie structurale [IBS - UMR 5075]
Adam, Virgile [Auteur]
Institut de biologie structurale [IBS - UMR 5075]
Aquila, Andrew [Auteur]
Linac Coherent Light Source [LCLS]
Barends, Thomas R. M. [Auteur]
Max Planck Institute for Medical Research [Heidelberg]
Boutet, Sébastien [Auteur]
Linac Coherent Light Source [LCLS]
Byrdin, Martin [Auteur]
Institut de biologie structurale [IBS - UMR 5075 ]
Carbajo, Sergio [Auteur]
Linac Coherent Light Source [LCLS]
Mora, Eugenio De La [Auteur]
Institut de biologie structurale [IBS - UMR 5075 ]
Doak, R. Bruce [Auteur]
Max Planck Institute for Medical Research [Heidelberg]
Feliks, Mikolaj [Auteur]
Institut de biologie structurale [IBS - UMR 5075 ]
Fieschi, Franck [Auteur]
Institut de biologie structurale [IBS - UMR 5075 ]
Foucar, Lutz [Auteur]
Max Planck Institute for Medical Research [Heidelberg]
Guillon, Virginia [Auteur]
Institut de biologie structurale [IBS - UMR 5075]
Hilpert, Mario [Auteur]
Max Planck Institute for Medical Research [Heidelberg]
Hunter, Mark S. [Auteur]
Linac Coherent Light Source [LCLS]
Jakobs, Stefan [Auteur]
Department of NanoBiophotonics [Göttingen]
Koglin, Jason E. [Auteur]
Linac Coherent Light Source [LCLS]
Kovacsova, Gabriela [Auteur]
Max Planck Institute for Medical Research [Heidelberg]
Lane, Thomas J. [Auteur]
Linac Coherent Light Source [LCLS]
Lévy, Bernard [Auteur]
Institut de Chimie Physique [ICP]
Liang, Mengning [Auteur]
Linac Coherent Light Source [LCLS]
Nass, Karol [Auteur]
Max Planck Institute for Medical Research [Heidelberg]
Ridard, Jacqueline [Auteur]
Institut de Chimie Physique [ICP]
Robinson, Joseph S. [Auteur]
Linac Coherent Light Source [LCLS]
Roome, Christopher M. [Auteur]
Max Planck Institute for Medical Research [Heidelberg]
Ruckebusch, Cyril [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516
Seaberg, Matthew [Auteur]
Linac Coherent Light Source [LCLS]
Thepaut, Michel [Auteur]
Institut de biologie structurale [IBS - UMR 5075]
Cammarata, Marco [Auteur]
Université de Rennes [UR]
Demachy, Isabelle [Auteur]
Institut de Chimie Physique [ICP]
Field, Martin [Auteur]
Institut de biologie structurale [IBS - UMR 5075 ]
Shoeman, Robert L. [Auteur]
Max Planck Institute for Medical Research [Heidelberg]
Bourgeois, Dominique [Auteur]
Institut de biologie structurale [IBS - UMR 5075 ]
Colletier, Jacques-Philippe [Auteur]
Institut de biologie structurale [IBS - UMR 5075 ]
Schlichting, Ilme [Auteur]
Max Planck Institute for Medical Research [Heidelberg]
Weik, Martin [Auteur]
Institut de biologie structurale [IBS - UMR 5075 ]

Journal title :

Nature Chemistry

Volume number :

10

Pages :

31-37

Publication date :

2018-09

ISSN :

1755-4330

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Chromophores absorb light in photosensitive proteins and thereby initiate fundamental biological processes such as photosynthesis, vision and biofluorescence. An important goal in their understanding is the provision of ...
Show more >Chromophores absorb light in photosensitive proteins and thereby initiate fundamental biological processes such as photosynthesis, vision and biofluorescence. An important goal in their understanding is the provision of detailed structural descriptions of the ultrafast photochemical events that they undergo, in particular of the excited states that connect chemistry to biological function. Here we report on the structures of two excited states in the reversibly photoswitchable fluorescent protein rsEGFP2. We populated the states through femtosecond illumination of rsEGFP2 in its non-fluorescent off state and observed their build-up (within less than one picosecond) and decay (on the several picosecond timescale). Using an X-ray free-electron laser, we performed picosecond time-resolved crystallography and show that the hydroxybenzylidene imidazolinone chromophore in one of the excited states assumes a near-canonical twisted configuration halfway between the trans and cis isomers. This is in line with excited-state quantum mechanics/molecular mechanics and classical molecular dynamics simulations. Our new understanding of the structure around the twisted chromophore enabled the design of a mutant that displays a twofold increase in its off-to-on photoswitching quantum yield.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CNRS
ENSCL
Université de Lille

Collections :

• Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516

Research team(s) :

Dynamics, Nanoscopy & Chemometrics (DyNaChem)

Submission date :

2021-11-16T08:23:30Z
2024-02-21T07:18:42Z