Title :

Water content thresholds in glycerol/water system: Low- and high-wavenumber Raman spectroscopy study

Author(s) :

Starciuc, Tatiana [Auteur]
Guinet, Yannick [Auteur]
Hédoux, Alain [Auteur]
Shalaev, Evgenyi [Auteur]

Journal title :

Journal of Molecular Liquids

Volume number :

321

Pages :

114678

Publication date :

2021

English abstract : [en]

Water structure is the key in such diverse processes as protein folding, catalysis of chemical reactions, and stability of pharmaceuticals and biopharmaceuticals. In the current investigation, glycerol-water mixtures are ...
Show more >Water structure is the key in such diverse processes as protein folding, catalysis of chemical reactions, and stability of pharmaceuticals and biopharmaceuticals. In the current investigation, glycerol-water mixtures are studied by low- and high-wavenumber Raman spectroscopy. Three water content regions, which are separated by two water content thresholds (WT), WT1 at 6.1 ± 0.7 wt% water and WT2 at 18.6 ± 4.4 wt% water, are identified, based on the trends in the hydrogen bond strength and the cage effect. It is hypothesized that WT1 corresponds to a transition from predominantly unclustered water molecules to small water clusters, and is associated with facilitation of proton transfer as water content exceeds WT1. The WT2 is proposed to mark formation of large (>100 molecules) water clusters, which can form stable nucleus of hexagonal ice at lower temperatures. These findings, if confirmed for other polyhydroxycompounds, could facilitate development of novel pharmaceuticals and vaccines, and also contribute to fundamental understanding of mechanisms of cryo- and lyo-protection of proteins, viruses, and cells.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Non spécifiée

Administrative institution(s) :

Université de Lille
CNRS
INRA
ENSCL

Collections :

• Unité Matériaux et Transformations (UMET) - UMR 8207

Research team(s) :

Matériaux Moléculaires et Thérapeutiques

Submission date :

2022-01-28T09:16:49Z