Title :

Agroindustrial Wastes as a Support for the Immobilization of Lipase from Thermomyces lanuginosus: Synthesis of Hexyl Laurate

Author(s) :

K. de S. Lira, Regiane [Auteur]
Universidade Federal Rural do Rio de Janeiro [UFRRJ]
T. Zardini, Rochele [Auteur]
Universidade Federal Rural do Rio de Janeiro [UFRRJ]
C. C. de Carvalho, Marcela [Auteur]
Universidade Federal Rural do Rio de Janeiro [UFRRJ]
Wojcieszak, Robert [Auteur]
Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181
G. F. Leite, Selma [Auteur]
Universidade Federal Rural do Rio de Janeiro [UFRRJ]
Itabaiana, Ivaldo [Auteur]
Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181

Journal title :

Biomolecules

Abbreviated title :

Biomolecules

Volume number :

11

Pages :

445

Publisher :

MDPI

Publication date :

2021-03-17

ISSN :

2218-273X

HAL domain(s) :

Chimie/Catalyse

English abstract : [en]

As a consequence of intense industrialization in the last few decades, the amount of agro-industrial wastes has increasing, where new forms of valorization are crucial. In this work, five residual biomasses from Maranhão ...
Show more >As a consequence of intense industrialization in the last few decades, the amount of agro-industrial wastes has increasing, where new forms of valorization are crucial. In this work, five residual biomasses from Maranhão (Brazil) were investigated as supports for immobilization of lipase from Thermomyces lanuginosus (TLL). The new biocatalysts BM-TLL (babaçu mesocarp) and RH-TLL (rice husk) showed immobilization efficiencies >98% and hydrolytic activities of 5.331 U g−1 and 4.608 U g−1, respectively, against 142 U g−1 by Lipozyme® TL IM. High esterification activities were also found, with 141.4 U g−1 and 396.4 U g−1 from BM-TLL and RH-TLL, respectively, against 113.5 U g−1 by TL IM. Results of porosimetry, SEM, and BET demonstrated BM and RH supports are mesoporous materials with large hydrophobic area, allowing a mixture of hydrophobic adsorption and confinement, resulting in hyperactivation of TLL. These biocatalysts were applied in the production of hexyl laurate, where RH-TLL was able to generate 94% conversion in 4 h. Desorption with Triton X-100 and NaCl confirmed that new biocatalysts were more efficient with 5 times less protein than commercial TL IM. All results demonstrated that residual biomass was able to produce robust and stable biocatalysts containing immobilized TLL with better results than commercial preparations.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CNRS
Centrale Lille
ENSCL
Univ. Artois
Université de Lille

Collections :

• Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181

Research team(s) :

Valorisation des alcanes et de la biomasse (VAALBIO)

Submission date :

2022-03-24T09:02:40Z
2023-01-11T09:54:48Z