Development of a qPCR method for the ...
Document type :
Article dans une revue scientifique: Article original
DOI :
PMID :
Title :
Development of a qPCR method for the identification and quantification of two closely related Tuna species, Bigeye Tuna (<I>Thunnus obesus</i>) and Yellowfin Tuna (<i>Thunnus albacares</i>), in canned Tuna
Author(s) :
Bojolly, Dahne [Auteur]
Centre National de la Recherche Scientifique [CNRS]
Université du Littoral Côte d'Opale [ULCO]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Doyen, Perine [Auteur]
Université d'Artois [UA]
Université de Lille
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Institut Supérieur d'Agriculture de Lille [ISA]
Institut National de la Recherche Agronomique [INRA]
Université du Littoral Côte d'Opale [ULCO]
Le Fur, Bruno [Auteur]
Christaki, Urania [Auteur]
Centre National de la Recherche Scientifique [CNRS]
Université du Littoral Côte d'Opale [ULCO]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Verrez Bagnis, Véronique [Auteur]
Unité Biotechnologies et Ressources Marines [BRM]
Grard, Thierry [Auteur]
Université de Lille
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Institut Supérieur d'Agriculture de Lille [ISA]
Institut National de la Recherche Agronomique [INRA]
Université d'Artois [UA]
Université du Littoral Côte d'Opale [ULCO]
Centre National de la Recherche Scientifique [CNRS]
Université du Littoral Côte d'Opale [ULCO]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Doyen, Perine [Auteur]
Université d'Artois [UA]
Université de Lille
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Institut Supérieur d'Agriculture de Lille [ISA]
Institut National de la Recherche Agronomique [INRA]
Université du Littoral Côte d'Opale [ULCO]
Le Fur, Bruno [Auteur]
Christaki, Urania [Auteur]
Centre National de la Recherche Scientifique [CNRS]
Université du Littoral Côte d'Opale [ULCO]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Verrez Bagnis, Véronique [Auteur]
Unité Biotechnologies et Ressources Marines [BRM]
Grard, Thierry [Auteur]

Université de Lille
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Institut Supérieur d'Agriculture de Lille [ISA]
Institut National de la Recherche Agronomique [INRA]
Université d'Artois [UA]
Université du Littoral Côte d'Opale [ULCO]
Journal title :
Journal of Agricultural and Food Chemistry
Pages :
913-920
Publisher :
American Chemical Society
Publication date :
2017-01-13
ISSN :
0021-8561
English keyword(s) :
tuna
authentication
quantification
canned products
TaqMan
qPCR
species identification
bigeye tuna (Thunnus obesus)
yellowfin tuna (Thunnus albacares)
authentication
quantification
canned products
TaqMan
qPCR
species identification
bigeye tuna (Thunnus obesus)
yellowfin tuna (Thunnus albacares)
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European ...
Show more >Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, as juveniles of bigeye and yellowfin tunas are very difficult to distinguish, unintentional substitutions may occur during the canning process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 and cytochrome c oxidase subunit II genes were used to identify bigeye tuna and yellowfin tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one was based on absolute quantification using standard curves realized with these two markers; the second one was founded on relative quantification with the universal 12S rRNA gene as the endogenous gene. On the basis of our results, we conclude that our methodology could be applied to authenticate these two closely related tuna species when used in a binary mix in tuna cans.Show less >
Show more >Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, as juveniles of bigeye and yellowfin tunas are very difficult to distinguish, unintentional substitutions may occur during the canning process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 and cytochrome c oxidase subunit II genes were used to identify bigeye tuna and yellowfin tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one was based on absolute quantification using standard curves realized with these two markers; the second one was founded on relative quantification with the universal 12S rRNA gene as the endogenous gene. On the basis of our results, we conclude that our methodology could be applied to authenticate these two closely related tuna species when used in a binary mix in tuna cans.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Non spécifiée
Popular science :
Non
Source :
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