Title :

Fruit fly phylogeny imprints bacterial gut microbiota

Author(s) :

Ravigné, Virginie [Auteur correspondant]
Plant Health Institute of Montpellier [UMR PHIM]
Becker, Nathalie [Auteur]
Institut de Systématique, Evolution, Biodiversité [ISYEB ]
Massol, Francois [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Guichoux, Erwan [Auteur]
Biodiversité, Gènes & Communautés [BioGeCo]
Boury, Christophe [Auteur]
Biodiversité, Gènes & Communautés [BioGeCo]
Mahé, Frédéric [Auteur]
Plant Health Institute of Montpellier [UMR PHIM]
Facon, Benoit [Auteur]
Centre de Biologie pour la Gestion des Populations [UMR CBGP]

Journal title :

Evolutionary Applications

Pages :

1621-1638

Publisher :

Blackwell

Publication date :

2022-10

ISSN :

1752-4563

English keyword(s) :

long-read sequencing
metabarcoding
community ecology

HAL domain(s) :

Sciences du Vivant [q-bio]/Biologie végétale

English abstract : [en]

One promising avenue for reconciling the goals of crop production and ecosystem preservation consists in the manipulation of beneficial biotic interactions, such as between insects and microbes. Insect gut microbiota can ...
Show more >One promising avenue for reconciling the goals of crop production and ecosystem preservation consists in the manipulation of beneficial biotic interactions, such as between insects and microbes. Insect gut microbiota can affect host fitness by contributing to development, host immunity, nutrition or behavior. However, the determinants of gut microbiota composition and structure, including host phylogeny and host ecology, remain poorly known. Here we used a well-studied community of eight sympatric fruit fly species to test the contributions of fly phylogeny, fly specialization, and fly sampling environment on the composition and structure of bacterial gut microbiota. Comprising both specialists and generalists, these species belong to five genera belonging to two tribes of the Tephritidae family. For each fly species, one field and one laboratory samples were studied. Bacterial inventories to the genus level were produced using 16S metabarcoding with the Oxford Nanopore technology. Sample bacterial compositions were analyzed with recent network-based clustering techniques. Whereas gut microbiota were dominated by the Enterobacteriacaeae family in all samples, microbial profiles varied across samples, mainly in relation with fly identity and sampling environment. Alpha diversity varied across samples and was higher in the Dacinae tribe than in the Ceratitinae tribe. Network analyses allowed grouping samples according to their microbial profiles. The resulting groups were very congruent with fly phylogeny, with a significant modulation of sampling environment, and a very low impact of fly specialization. Such a strong imprint of host phylogeny in sympatric fly species, some of which share much of their host plants, suggests important control of fruit flies on their gut microbiota through vertical transmission and/or intense filtering of environmental bacteria.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

ANR Project :

Biosurveillance Next-Gen des changements dans la structure et le fonctionnement des écosystèmes

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL