Long-Term Energy Deficit in Mice Causes ...
Type de document :
Article dans une revue scientifique: Article original
PMID :
URL permanente :
Titre :
Long-Term Energy Deficit in Mice Causes Long-Lasting Hypothalamic Alterations after Recovery.
Auteur(s) :
Méquinion, Mathieu [Auteur]
Le Thuc, Ophélia [Auteur]
Zgheib, Sara [Auteur]
Alexandre, David [Auteur]
Chartrel, Nicolas [Auteur]
Rovère, Carole [Auteur]
Hardouin, Pierre [Auteur]
Viltart, Odile [Auteur]
Chauveau, Christophe [Auteur]
Le Thuc, Ophélia [Auteur]
Zgheib, Sara [Auteur]
Alexandre, David [Auteur]
Chartrel, Nicolas [Auteur]
Rovère, Carole [Auteur]
Hardouin, Pierre [Auteur]
Viltart, Odile [Auteur]
Chauveau, Christophe [Auteur]
Titre de la revue :
Neuroendocrinology
Nom court de la revue :
Neuroendocrinology
Numéro :
105
Pagination :
372-383
Date de publication :
2017
ISSN :
1423-0194
Mot(s)-clé(s) en anglais :
Agouti-Related Protein
Animals
Body Weight
Cytokines
Disease Models, Animal
Eating
Female
Hypolipoproteinemias
Hypothalamic Hormones
Hypothalamus
Leptin
Melanins
Mice
Mice, Inbred C57BL
Neuropeptide Y
Neuropeptides
Orexins
Pituitary Hormones
RNA, Messenger
Receptors, Leptin
Receptors, Neuropeptide
Animal model
Anorexia nervosa
Anorexigenic peptides
Hypothalamus
Leptin
Long-term energy deficit
Orexigenic peptides
Refeeding
Animals
Body Weight
Cytokines
Disease Models, Animal
Eating
Female
Hypolipoproteinemias
Hypothalamic Hormones
Hypothalamus
Leptin
Melanins
Mice
Mice, Inbred C57BL
Neuropeptide Y
Neuropeptides
Orexins
Pituitary Hormones
RNA, Messenger
Receptors, Leptin
Receptors, Neuropeptide
Animal model
Anorexia nervosa
Anorexigenic peptides
Hypothalamus
Leptin
Long-term energy deficit
Orexigenic peptides
Refeeding
Résumé en anglais : [en]
Although the short-term effects of fasting or energy deficit on hypothalamic neuropeptide circuitries are now better understood, the effects of long-term energy deficit and refeeding remain to be elucidated. We showed that ...
Lire la suite >Although the short-term effects of fasting or energy deficit on hypothalamic neuropeptide circuitries are now better understood, the effects of long-term energy deficit and refeeding remain to be elucidated. We showed that after a long-term energy deficit, mice exhibited persistent hypoleptinemia following the refeeding period despite restoration of fat mass, ovarian activity, and feeding behavior. We aimed to examine the hypothalamic adaptations after 10 weeks of energy deficit and after 10 further weeks of nutritional recovery. To do so, we assessed the mRNA levels of the leptin receptor and the main orexigenic and anorexigenic peptides, and their receptors regulated by leptin. Markers of hypothalamic inflammation were assessed as leptin can also participate in this phenomenon. Long-term time-restricted feeding and separation induced significant increase in mRNA levels of hypothalamic orexigenic peptides, while both Y1 and Y5 receptor mRNAs were downregulated. No changes occurred in the mRNA levels of orexin (OX), melanin-concentrating hormone, pro-opiomelanocortin, 26RFa (26-amino acid RF-amide peptide), and their receptors despite an increase in the expression of melanocortin receptors (MC3-R and MC4-R) and OXR1 (OX receptor 1). The refeeding period induced an overexpression of leptin receptor mRNA in the hypothalamus. The other assessed mRNA levels were normalized except for Y2, Y5, MC3-R, and MC4-R, which remained upregulated. No convincing changes were observed in neuroinflammatory markers, even if interleukin-1β mRNA levels were increased in parallel with those of Iba1 (ionized calcium-binding adaptor molecule 1), a marker of microglial activation. Normalization of leptin-regulated functions and hypothalamic gene expressions in refed mice with low plasma leptin levels could be sustained by recalibration of hypothalamic sensitivity to leptin.Lire moins >
Lire la suite >Although the short-term effects of fasting or energy deficit on hypothalamic neuropeptide circuitries are now better understood, the effects of long-term energy deficit and refeeding remain to be elucidated. We showed that after a long-term energy deficit, mice exhibited persistent hypoleptinemia following the refeeding period despite restoration of fat mass, ovarian activity, and feeding behavior. We aimed to examine the hypothalamic adaptations after 10 weeks of energy deficit and after 10 further weeks of nutritional recovery. To do so, we assessed the mRNA levels of the leptin receptor and the main orexigenic and anorexigenic peptides, and their receptors regulated by leptin. Markers of hypothalamic inflammation were assessed as leptin can also participate in this phenomenon. Long-term time-restricted feeding and separation induced significant increase in mRNA levels of hypothalamic orexigenic peptides, while both Y1 and Y5 receptor mRNAs were downregulated. No changes occurred in the mRNA levels of orexin (OX), melanin-concentrating hormone, pro-opiomelanocortin, 26RFa (26-amino acid RF-amide peptide), and their receptors despite an increase in the expression of melanocortin receptors (MC3-R and MC4-R) and OXR1 (OX receptor 1). The refeeding period induced an overexpression of leptin receptor mRNA in the hypothalamus. The other assessed mRNA levels were normalized except for Y2, Y5, MC3-R, and MC4-R, which remained upregulated. No convincing changes were observed in neuroinflammatory markers, even if interleukin-1β mRNA levels were increased in parallel with those of Iba1 (ionized calcium-binding adaptor molecule 1), a marker of microglial activation. Normalization of leptin-regulated functions and hypothalamic gene expressions in refed mice with low plasma leptin levels could be sustained by recalibration of hypothalamic sensitivity to leptin.Lire moins >
Langue :
Anglais
Audience :
Non spécifiée
Établissement(s) :
Université de Lille
CNRS
CHU Lille
CNRS
CHU Lille
Date de dépôt :
2022-05-06T14:57:28Z
Fichiers
- Méquinion et al. 2016 Neuroendoc.pdf
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